Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Sci Rep. 2023 Feb 15;13(1):2716. doi: 10.1038/s41598-023-29918-w.
To date, the impossibility of treating resistant forms of bacteria and fungi (AMR) with traditional drugs is a cause for global alarm. We have made the green synthesis of Argirium silver ultra nanoclusters (Argirium-SUNCs) very effective against resistant bacteria (< 1 ppm) and mature biofilm (0.6 ppm). In vitro and preclinical tests indicate that SUNCs are approximately 10 times less toxic in human cells than bacteria. Unique chemical-physical characteristics such as particle size < 2 nm, a core composed of Ag, and a shell of Ag , Ag , Ag never observed before in stable form in ultra pure water, explain their remarkable redox properties Otto Cars (Lancet Glob. Health 9:6, 2021). Here we show that Argirium-SUNCs have strong antimicrobial properties also against resistant Aspergillus niger GM31 mycelia and spore inactivation (0.6 ppm). The membrane depolarization is a primary target leading to cell death as already observed in bacteria. Being effective against both bacteria and fungi Argirium-SUNCs represent a completely different tool for the treatment of infectious diseases.
迄今为止，用传统药物治疗耐药形式的细菌和真菌（AMR）的可能性是引发全球警报的原因。我们已经非常有效地进行了银纳米簇（Argirium-SUNCs）的绿色合成，该合成对耐药细菌（<1ppm）和成熟生物膜（0.6ppm）非常有效。体外和临床前测试表明，SUNCs 在人类细胞中的毒性比细菌低约 10 倍。独特的理化特性，如粒径<2nm、由 Ag 组成的核和以前从未在超纯水中以稳定形式观察到的 Ag、Ag、Ag 壳，解释了它们显著的氧化还原特性 Otto Cars（柳叶刀全球健康 9：6,2021）。在这里，我们表明 Argirium-SUNCs 对耐药黑曲霉 GM31 菌丝体和孢子失活（0.6ppm）也具有很强的抗菌特性。膜去极化是导致细胞死亡的主要靶点，正如已经在细菌中观察到的那样。Argirium-SUNCs 对细菌和真菌均有效，代表了治疗传染病的一种完全不同的工具。
