Salem Salem S, Ali Omar M, Reyad Ahmed M, Abd-Elsalam Kamel A, Hashem Amr H
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Department of Chemistry, Turabah University College, Turabah Branch, Taif University, Taif 21944, Saudi Arabia.
J Fungi (Basel). 2022 Jan 27;8(2):126. doi: 10.3390/jof8020126.
Mucormycosis is considered one of the most dangerous invasive fungal diseases. In this study, a facile, green and eco-friendly method was used to biosynthesize silver nanoparticles (AgNPs) using S. Azhar, to combat fungi causing mucormycosis. The biosynthesis of AgNPs was validated by a progressive shift in the color of filtrate from colorless to brown, as well as the identification of a distinctive absorption peak at 420 nm using UV-vis spectroscopy. Fourier-transform infrared spectroscopy (FTIR) results indicated the existence of bioactive chemicals that are responsible for AgNP production. AgNPs with particle sizes ranging from 2.4 to 53.5 nm were discovered using transmission electron microscopy (TEM). Pattern peaks corresponding to the 111, 200, 220, 311, and 222 planes, which corresponded to face-centered cubic forms of metallic silver, were also discovered using X-ray diffraction (XRD). Moreover, antifungal activity measurements of biosynthesized AgNPs against , and were carried out. Results of antifungal activity analysis revealed that the biosynthesized AgNPs exhibited outstanding antifungal activity against all tested fungi at a concentration of 400 µg/mL, where minimum inhibitory concentrations (MIC) were 50, 50, and 100 µg/mL toward , and respectively. In addition, the biosynthesized AgNPs revealed antioxidant activity, where IC was 31 µg/mL when compared to ascorbic acid (0.79 µg/mL). Furthermore, the biosynthesized AgNPs showed no cytotoxicity on the Vero normal cell line. In conclusion, the biosynthesized AgNPs in this study can be used as effective antifungals with safe use, particularly for fungi causing mucormycosis.
毛霉菌病被认为是最危险的侵袭性真菌疾病之一。在本研究中，采用了一种简便、绿色且环保的方法，利用阿扎尔链霉菌生物合成银纳米颗粒（AgNPs），以对抗引起毛霉菌病的真菌。AgNPs的生物合成通过滤液颜色从无色逐渐变为棕色得到验证，同时利用紫外可见光谱在420nm处鉴定出一个独特的吸收峰。傅里叶变换红外光谱（FTIR）结果表明存在负责AgNP产生的生物活性化学物质。使用透射电子显微镜（TEM）发现粒径范围为2.4至53.5nm的AgNPs。还利用X射线衍射（XRD）发现了与金属银的面心立方形式相对应的111、200、220、311和222晶面的衍射峰。此外，还对生物合成的AgNPs针对[此处原文缺失具体真菌名称]、[此处原文缺失具体真菌名称]和[此处原文缺失具体真菌名称]进行了抗真菌活性测定。抗真菌活性分析结果显示，生物合成的AgNPs在浓度为400μg/mL时对所有测试真菌均表现出出色的抗真菌活性，其中对[此处原文缺失具体真菌名称]、[此处原文缺失具体真菌名称]和[此处原文缺失具体真菌名称]的最小抑菌浓度（MIC）分别为50、50和100μg/mL。此外，生物合成的AgNPs显示出抗氧化活性，与抗坏血酸（0.79μg/mL）相比，其半数抑制浓度（IC）为31μg/mL。此外，生物合成的AgNPs对Vero正常细胞系没有细胞毒性。总之，本研究中生物合成的AgNPs可用作有效的抗真菌剂，使用安全，特别是针对引起毛霉菌病的真菌。
