Gansu University of Traditional Chinese Medicine, Lanzhou, 730000, China.
Chem Biodivers. 2023 Mar;20(3):e202200813. doi: 10.1002/cbdv.202200813. Epub 2023 Feb 15.
Angelica Sinensis polysaccharide cerium (ASP-Ce) was prepared by Angelica Sinensis polysaccharide (ASP) and cerium ammonium nitrate (NH ) Ce(NO ) . and its morphology, and solid structure was investigated. The antioxidant activity of the ASP-Ce complex in vitro was evaluated. The antioxidant activity of ASP-Ce complex in vitro was evaluated by the scavenging activity of 2,2-diphenyl-1-picrylhyrazyl radical (DPPH), hydroxyl radical (⋅OH) and superoxide anion radical ( ⋅). The results showed that the ASP-Ce had the more ordered structure for inserting the Ce into the polymer chain of ASP and there was little change in the conformation of the polysaccharide from Ce . Three free radical scavenging experiments proved that ASP-Ce had better antioxidant capacity than of ASP, especially on DPPH, and then on ⋅. The scavenging rate of ASP-Ce at 1.0 mg/mL on DPPH reached 71.6 %. Therefore, these results provide references for the further development and utilization of rare earth-polysaccharide.
当归多糖铈(ASP-Ce)由当归多糖(ASP)和硝酸铈铵((NH ) Ce(NO ) 制备。对其形态和固体结构进行了研究。体外评价了 ASP-Ce 配合物的抗氧化活性。通过 2,2-二苯基-1-苦基肼自由基(DPPH)、羟基自由基(⋅OH)和超氧阴离子自由基( ⋅)的清除活性来评价 ASP-Ce 配合物的体外抗氧化活性。结果表明,ASP-Ce 将 Ce 插入 ASP 聚合物链中具有更有序的结构,并且多糖的构象从 Ce 后几乎没有变化。三种自由基清除实验证明,ASP-Ce 具有比 ASP 更好的抗氧化能力,特别是对 DPPH,然后是 ⋅。ASP-Ce 在 1.0 mg/mL 时对 DPPH 的清除率达到 71.6%。因此,这些结果为进一步开发和利用稀土-多糖提供了参考。