Yao Xiaohui, Song Qiangqiang, Zhu Wei, Wei Jianchao, Shao Donghua, Liu Ke, Li Zongjie, Qiu Yafeng, Ma Zhiyong, Xia Lining, Li Beibei
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
College of Veterinary Medicine, Xinjiang Agricultural University, Urmuqi, China.
Front Vet Sci. 2023 Jan 30;10:1084491. doi: 10.3389/fvets.2023.1084491. eCollection 2023.
and are two important bacterial pathogens in swine industry. In the present study, resistance profiles of nine commonly used antibiotics of . and . isolates of swine origin from different regions of China were investigated by determination of minimum inhibitory concentrations (MICs). In addition, genetic relationship of the florfenicol-resistant . and . isolates was determined by pulsed-field gel electrophoresis (PFGE). The genetic basis of florfenicol resistance in these isolates were explored by detection and whole genome sequencing. High resistance rates (>25%) of florfenicol, tetracycline and trimethoprim- sulfamethoxazole were observed for both bacteria. No ceftiofur- and tiamulin- resistant isolates were detected. Furthermore, all the 17 florfenicol-resistant isolates (nine for . and eight for . ) were positive for gene. The presence of similar PFGE types in these isolates suggested that clonal expansion of some -producing strains occurred in the pig farms from same regions. WGS and PCR screening showed that three plasmids, named pFA11, pMAF5, and pMAF6, were the cargos of the genes in the 17 isolates. Plasmid pFA11 exhibited novel structure and carried several resistance genes, including , and . Plasmids pMAF5 and pMAF6 were presented in . and . isolates from different regions, suggesting horizontal transfer of the two plasmids are important for the dissemination in these pathogens. Further studies of florfenicol resistance and its transfer vectors in bacteria of veterinary origin are warranted.
[细菌名称1]和[细菌名称2]是养猪业中两种重要的细菌病原体。在本研究中，通过测定最小抑菌浓度（MIC），调查了来自中国不同地区猪源的[细菌名称1]和[细菌名称2]分离株对九种常用抗生素的耐药谱。此外，通过脉冲场凝胶电泳（PFGE）确定了氟苯尼考耐药的[细菌名称1]和[细菌名称2]分离株的遗传关系。通过[检测方法]检测和全基因组测序探索了这些分离株中氟苯尼考耐药的遗传基础。两种细菌对氟苯尼考、四环素和甲氧苄啶-磺胺甲恶唑均表现出高耐药率（>25%）。未检测到对头孢噻呋和替米考星耐药的分离株。此外，所有17株氟苯尼考耐药分离株（[细菌名称1]9株，[细菌名称2]8株）的[基因名称]基因均为阳性。这些分离株中存在相似的PFGE类型，表明在同一地区的猪场中发生了一些[产生物质名称]产生菌株的克隆扩增。全基因组测序和PCR筛选表明，三种质粒，命名为pFA11、pMAF5和pMAF6，是17株分离株中[基因名称]基因的载体。质粒pFA11呈现出新颖的结构，并携带多个耐药基因，包括[耐药基因名称1]、[耐药基因名称2]和[耐药基因名称3]。质粒pMAF5和pMAF6存在于来自不同地区的[细菌名称1]和[细菌名称2]分离株中，表明这两种质粒的水平转移对于这些[细菌名称]病原体中[基因名称]的传播很重要。有必要对兽源[细菌名称]中氟苯尼考耐药性及其转移载体进行进一步研究。
