Huang Debao, Gao Liwei, McAdams Jeremy, Zhao Fangzhou, Lu Hongyan, Wu Yonghui, Martin Jeremy, Sherif Sherif M, Subramanian Jayasankar, Duan Hui, Liu Wusheng
Department of Horticultural Science, North Carolina State University, Raleigh, NC 27607, USA.
College of Life Sciences, Ganzhou Normal University, Ganzhou, Jiangxi 341000, China.
Hortic Res. 2022 Dec 22;10(2):uhac280. doi: 10.1093/hr/uhac280. eCollection 2023 Feb.
is a self-pollinating and facultative outcrossing oilseed crop. Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition, modified protein profiles, improved seed and oil yield, and enhanced drought resistance. The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into non-transgenic camelina and wild relatives. Thus, effective bioconfinement strategies need to be developed to prevent pollen-mediated gene flow (PMGF) from transgenic camelina. In the present study, we overexpressed the cleistogamy (i.e. floral petal non-openness)-inducing gene from peach in transgenic camelina. Transgenic camelina overexpressing showed three levels of cleistogamy, affected pollen germination rates after anthesis but not during anthesis, and caused a minor silicle abortion only on the main branches. We also conducted field trials to examine the effects of the overexpressed on PMGF in the field, and found that the overexpressed dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions. Thus, the engineered cleistogamy using the overexpressed is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina, and could be used for bioconfinement in other dicot species.
是一种自花授粉且兼性异交的油料作物。基因工程已被用于提高荠蓝的产量潜力，以改变脂肪酸组成、改良蛋白质谱、提高种子和油的产量以及增强抗旱性。在田间种植转基因荠蓝存在与转基因渗入非转基因荠蓝和野生近缘种相关的高风险。因此，需要制定有效的生物限制策略来防止转基因荠蓝通过花粉介导的基因流动（PMGF）。在本研究中，我们在转基因荠蓝中过表达了来自桃子的闭花受精（即花瓣不开放）诱导基因。过表达的转基因荠蓝表现出三个闭花受精水平，影响花后但不影响花期的花粉萌发率，并且仅在主枝上导致轻微的角果败育。我们还进行了田间试验，以研究过表达的该基因对田间PMGF的影响，发现在田间条件下，过表达的该基因显著抑制了转基因荠蓝向非转基因荠蓝的PMGF。因此，利用过表达的该基因构建的闭花受精是一种限制转基因荠蓝PMGF的高效生物限制策略，并且可用于其他双子叶物种的生物限制。
