Lu Chaofu, Kang Jinling
Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA.
Plant Cell Rep. 2008 Feb;27(2):273-8. doi: 10.1007/s00299-007-0454-0. Epub 2007 Sep 27.
Camelina sativa is an alternative oilseed crop that can be used as a potential low-cost biofuel crop or a source of health promoting omega-3 fatty acids. Currently, the fatty acid composition of camelina does not uniquely fit any particular uses, thus limit its commercial value and large-scale production. In order to improve oil quality and other agronomic characters, we have developed an efficient and simple in planta method to generate transgenic camelina plants. The method included Agrobacterium-mediated inoculation of plants at early flowering stage along with a vacuum infiltration procedure. We used a fluorescent protein (DsRed) as a visual selection marker, which allowed us to conveniently screen mature transgenic seeds from a large number of untransformed seeds. Using this method, over 1% of transgenic seeds can be obtained. Genetic analysis revealed that most of transgenic plants contain a single copy of transgene. In addition, we also demonstrated that transgenic camelina seeds produced novel hydroxy fatty acids by transforming a castor fatty acid hydroxylase. In conclusion, our results provide a rapid means to genetically improve agronomic characters of camelina, including fatty acid profiles of its seed oils. Camelina may serve as a potential industrial crop to produce novel biotechnology products.
亚麻荠是一种可供选择的油料作物，可用作潜在的低成本生物燃料作物或促进健康的ω-3脂肪酸来源。目前，亚麻荠的脂肪酸组成并不完全适合任何特定用途，因此限制了其商业价值和大规模生产。为了提高油质和其他农艺性状，我们开发了一种高效且简单的在植物体内产生转基因亚麻荠植株的方法。该方法包括在开花初期通过农杆菌介导接种植株并结合真空渗透程序。我们使用荧光蛋白（DsRed）作为视觉选择标记，这使我们能够方便地从大量未转化的种子中筛选出成熟的转基因种子。使用这种方法，可以获得超过1%的转基因种子。遗传分析表明，大多数转基因植株含有单个转基因拷贝。此外，我们还证明了通过转化蓖麻脂肪酸羟化酶，转基因亚麻荠种子产生了新型羟基脂肪酸。总之，我们的结果提供了一种快速的方法来遗传改良亚麻荠的农艺性状，包括其种子油的脂肪酸谱。亚麻荠可能成为生产新型生物技术产品的潜在工业作物。
