Jia Yaqi, Zhao Huimin, Niu Yani, Wang Yucheng
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Key Laboratory of Forest Tree Genetic Breeding and Cultivation of Liaoning Province, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China.
Hortic Res. 2022 Dec 9;10(2):uhac277. doi: 10.1093/hr/uhac277. eCollection 2023 Feb.
Long noncoding RNAs (lncRNAs) are important in abiotic stress tolerance. Here, we identified salt-responsive genes and lncRNAs in the roots and leaves of Suk. (birch), and characterized their lncRNAs functions. In total, 2660 mRNAs and 539 lncRNAs responding to salt treatment were identified using RNA-seq. The salt-responsive genes were substantially enriched in 'cell wall biogenesis' and 'wood development' in the roots and were enriched in 'photosynthesis' and 'response to stimulus' in the leaves. Meanwhile, the potential target genes of the salt-responsive lncRNAs in roots and leaves were both enriched in 'nitrogen compound metabolic process' and 'response to stimulus'. We further built a method for quickly identifying abiotic stress tolerance of lncRNAs, which employed transient transformation for overexpression and knock-down of the lncRNA, enabling gain- and loss-of-function analysis. Using this method, 11 randomly selected salt-responsive lncRNAs were characterized. Among them, six lncRNAs confer salt tolerance, two lncRNAs confer salt sensitivity, and the other three lncRNAs are not involved in salt tolerance. In addition, a lncRNA, , was further characterized, which improves salt tolerance by regulating two transcription factors, and . Taken together, our results suggested that lncRNAs play important roles in the salt response of birch plants.
长链非编码RNA（lncRNAs）在非生物胁迫耐受性中起着重要作用。在此，我们鉴定了 Suk.（桦树）根和叶中的盐响应基因和lncRNAs，并对其lncRNAs功能进行了表征。使用RNA测序共鉴定出2660个响应盐处理的mRNA和539个lncRNAs。盐响应基因在根中大量富集于“细胞壁生物合成”和“木材发育”，在叶中富集于“光合作用”和“对刺激的响应”。同时，根和叶中盐响应lncRNAs的潜在靶基因均富集于“氮化合物代谢过程”和“对刺激的响应”。我们进一步建立了一种快速鉴定lncRNAs非生物胁迫耐受性的方法，该方法采用瞬时转化来过表达和敲低lncRNA，实现功能获得和功能缺失分析。使用该方法，对11个随机选择的盐响应lncRNAs进行了表征。其中，6个lncRNAs赋予耐盐性，2个lncRNAs赋予盐敏感性，另外3个lncRNAs不参与耐盐性。此外，对一个lncRNA， 进行了进一步表征，它通过调节两个转录因子 和 来提高耐盐性。综上所述，我们的结果表明lncRNAs在桦树植物的盐响应中发挥重要作用。
