Zhang Yingting, Yan Qingyu, Xia Hui, Zeng Xiangling, Yang Jie, Cai Xuan, Li Zeqing, Chen Hongguo, Zou Jingjing
National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China.
Front Plant Sci. 2025 Jan 21;15:1492854. doi: 10.3389/fpls.2024.1492854. eCollection 2024.
, a well-regarded traditional flower in China, holds extensive applications in gardening, food, cosmetics, and traditional Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) in has been constrained by the absence of reliable reference genes (RGs).
We employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions.
The results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194; drought stress: lnc00042194 and lnc00174850; salt stress: lnc00239991 and lnc00042194; abiotic stress: lnc00239991, lnc00042194, lnc00067193, and lnc00265419; ABA treatment: lnc00239991 and ; MeJA treatment: lnc00265419 and lnc00249739; ethephon treatment: lnc00229717 and lnc00044331; hormone treatments: lnc00265419 and lnc00239991; Al treatment: lnc00087780 and lnc00265419; Cu treatment: lnc00067193 and ; Fe treatment: lnc00229717 and ; metal ion treatment: lnc00239991 and lnc00067193; flowering stage: lnc00229717 and ; different tissues: lnc00239991, lnc00042194, lnc00067193, , , and ; and across all samples: lnc00239991, lnc00042194, lnc00265419 and . The reliability of these selected RGs was further validated by analyzing the expression patterns of lnc00003036, lnc00126603, and lnc00250780. This study represents the first comprehensive evaluation of lncRNA RG stability in , significantly enhancing the accuracy and depth of lncRNA expression analyses in this species, contributing to advancements in plant stress resistance breeding and improving environmental adaptability.
[植物名称]是中国一种备受推崇的传统花卉，在园艺、食品、化妆品和传统中药领域有广泛应用。尽管其很重要，但由于缺乏可靠的内参基因（RGs），对[植物名称]中长链非编码RNA（lncRNAs）的研究受到了限制。
我们采用了五种不同的算法，即delta-Ct、NormFinder、geNorm、BestKeeper和RefFinder，来评估17个候选内参基因在各种实验条件下的表达稳定性。
结果表明，不同条件下最稳定的内参基因组合如下：冷胁迫：lnc00249739和lnc00042194；干旱胁迫：lnc00042194和lnc00174850；盐胁迫：lnc00239991和lnc00042194；非生物胁迫：lnc00239991、lnc00042194、lnc00067193和lnc00265419；脱落酸处理：lnc00239991和[基因名称缺失]；茉莉酸甲酯处理：lnc00265419和lnc00249739；乙烯利处理：lnc00229717和lnc00044331；激素处理：lnc00265419和lnc00239991；铝处理：lnc00087780和lnc00265419；铜处理：lnc00067193和[基因名称缺失]；铁处理：lnc00229717和[基因名称缺失]；金属离子处理：lnc00239991和lnc00067193；开花期：lnc00229717和[基因名称缺失]；不同组织：lnc00239991、lnc00042194、lnc00067193、[基因名称缺失]、[基因名称缺失]和[基因名称缺失]；以及所有样本：lnc00239991、lnc00042194、lnc00265419和[基因名称缺失]。通过分析lnc00003036、lnc00126603和lnc00250780的表达模式，进一步验证了这些选定内参基因的可靠性。本研究是对[植物名称]中lncRNA内参基因稳定性的首次全面评估，显著提高了该物种中lncRNA表达分析的准确性和深度，有助于植物抗逆育种的进展并提高环境适应性。
