Liu Xiao-Lin, Chen Ming-Qing, Jiang Yang-Lin, Gao Rong-Yao, Wang Ze-Jun, Wang Peng
Department of Chemistry, Renmin University of China, Beijing, China.
Front Mol Biosci. 2023 Jan 30;10:1106832. doi: 10.3389/fmolb.2023.1106832. eCollection 2023.
The purple non-sulfur bacterium was selected as a biological model to investigate its response to the toxicity of 1-alkyl-3-methylimidazolium bromide ([Cmim]Br), a type of ionic liquid (IL), with different alkyl chain lengths ( describes the number of carbon atoms in the alkyl chain). The inhibition of bacterial growth by [Cmim]Br was positively correlated with . Morphological characterization revealed that [Cmim]Br caused cell membrane perforation. The signal amplitude of the electrochromic absorption band shift of endogenous carotenoids showed a negatively linear correlation with , and the amplitude of the blue-shift of the B850 band in light-harvesting complex 2 showed a positively linear correlation with . Furthermore, an increase in blocked ATP synthesis and increase in antioxidant enzyme activity were observed in chromatophores treated with ILs containing longer alkyl chains. In summary, the purple bacterium can be developed as a model to monitor ecotoxicity and examine the mechanism of IL toxicity.
紫色非硫细菌被选为生物模型，以研究其对1-烷基-3-甲基咪唑溴盐（[Cmim]Br）（一种离子液体（IL））毒性的反应，该离子液体具有不同的烷基链长度（表示烷基链中的碳原子数）。[Cmim]Br对细菌生长的抑制作用与呈正相关。形态学表征显示，[Cmim]Br导致细胞膜穿孔。内源性类胡萝卜素的电致变色吸收带位移的信号幅度与呈负线性相关，而光捕获复合物2中B850带的蓝移幅度与呈正线性相关。此外，在用含较长烷基链的离子液体处理的载色体中，观察到ATP合成受阻增加和抗氧化酶活性增加。总之，紫色细菌可被开发为监测生态毒性和研究离子液体毒性机制的模型。
