Rahman Mohammad Mojibur, Sabuj Abdullah Al Momen, Islam Md Saiful, Islam Md Alimul, Alam Jahangir, Ershaduzzaman Md, Saha Sukumar
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Bangladesh Civil Service Livestock Academy, Savar, Dhaka 1349, Bangladesh.
Saudi J Biol Sci. 2023 Mar;30(3):103565. doi: 10.1016/j.sjbs.2023.103565. Epub 2023 Jan 23.
A cross-sectional study was conducted to determine the seroprevalence of the Peste des Petits Ruminant (PPR) virus (PPRV) in sheep populations and to determine the potential epidemiological risk factors associated with this infection. Between October 2014 and March 2017, 2420 sheep serum samples were collected from ten selected PPR outbreak-prone districts in Bangladesh. The collected sera were analysed by competitive enzyme-linked immunosorbent assay (cELISA) test to detect antibodies against PPR. A previously designed disease report form was used to gather data on important epidemiological risk factors, and a risk analysis was performed to ascertain their association with PPRV infection. By cELISA, 44.3 % (95 % confidence interval:42.4-46.4 %) of sheep sera were positive for PPRV antibodies against PPR. In univariate analysis, the Bagerhat district had significantly higher seropositivity (54.1 %, 156/288) than other districts. Moreover, significantly higher ( < 0.05) seropositivity was found in the Jamuna River Basin (49.1 %, 217/442) compared to other ecological zones, in crossbreeds (60 %; 600/1000) related to native sheep, in males (69.8 %, 289/414) associated with females, in imported sheep (74.3 %, 223/300) compared to other sources, and in winter (57.2 %, 527/920) than in other seasons. In the multivariate logistic regression model, six possible risk factors were identified: study location, ecological zone, breed, sex, source, and season. The high seroprevalence of PPRV is significantly associated with several risk factors, suggesting that PPR is epizootic throughout the country.
开展了一项横断面研究，以确定绵羊群体中小反刍兽疫（PPR）病毒（PPRV）的血清流行率，并确定与这种感染相关的潜在流行病学风险因素。2014年10月至2017年3月期间，从孟加拉国10个选定的PPR易暴发地区采集了2420份绵羊血清样本。通过竞争酶联免疫吸附测定（cELISA）试验分析采集的血清，以检测抗PPR的抗体。使用先前设计的疾病报告表收集重要流行病学风险因素的数据，并进行风险分析以确定它们与PPRV感染的关联。通过cELISA，44.3%（95%置信区间：42.4 - 46.4%）的绵羊血清抗PPR的PPRV抗体呈阳性。在单变量分析中，巴盖尔哈德地区的血清阳性率（54.1%，156/288）显著高于其他地区。此外，与其他生态区相比，贾木纳河流域（49.1%，217/442）的血清阳性率显著更高（<0.05）；与本地绵羊相比，杂交品种（60%；600/1000）的血清阳性率显著更高；雄性（69.8%，289/414）与雌性相比血清阳性率显著更高；与其他来源相比，进口绵羊（74.3%，223/300）的血清阳性率显著更高；冬季（57.2%，527/920）的血清阳性率高于其他季节。在多变量逻辑回归模型中，确定了六个可能的风险因素：研究地点、生态区、品种、性别、来源和季节。PPRV的高血清流行率与几个风险因素显著相关，表明PPR在全国呈流行状态。
