Pathology department of Shanxi Medical University Fenyang College, No.16, Xueyuan Road, Fenyang City, 032200, Shanxi Province, China.
Pathology department Shanxi Fenyang Hospital, No.186, Shengli Road, Fenyang City, 032200, Shanxi Province, China.
Clin Transl Oncol. 2023 Jul;25(7):2239-2249. doi: 10.1007/s12094-023-03113-9. Epub 2023 Feb 16.
To determine whether ezrin regulates Yes-associated protein (YAP) and programed cell death ligand-1 (PD-L1), which are involved in the invasion and metastasis of non-small cell lung cancer (NSCLC).
Immunohistochemistry of 164 NSCLC and 16 para-cancer tissues was performed to detect ezrin, YAP, and PD-L1 expression. Further, H1299 and A549 cells were transfected with lentivirus, and then colony formation, CCK8, transwell, and wound-healing assays were used to assess cell proliferation, migration, and invasion. RT-qPCR and western blotting were used for quantitative analysis of ezrin, PD-L1, and YAP expression. Moreover, the role of ezrin in tumor growth was assessed in vivo, and immunohistochemistry and western blotting were performed to evaluate changes in ezrin expression in mouse samples.
The positive protein expression rates of these molecules in NSCLC were as follows: ezrin, 43.9% (72/164); YAP, 54.3% (89/164); and PD-L1, 47.6% (78/164); these were higher than those in normal lung tissues. Moreover, YAP and ezrin expression positively correlated with PD-L1 expression. Ezrin promoted proliferation, migration, invasion, and expression of YAP and PD-L1in NSCLC. Inhibition of ezrin expression reduced the effects of ezrin on cell proliferation, migration, invasion, inhibited the expression of YAP and PD-L1, and obviously reduced experimental tumor volume in vivo.
Ezrin is overexpressed in NSCLC patients and correlates with PD-L1 and YAP expression. Ezrin regulates YAP and PD-L1 expression. Inhibition of ezrin delayed NSCLC progression.
确定 ezrin 是否调节参与非小细胞肺癌（NSCLC）侵袭和转移的 Yes 相关蛋白（YAP）和程序性细胞死亡配体 1（PD-L1）。
对 164 例 NSCLC 组织和 16 例癌旁组织进行免疫组织化学检测，以检测 ezrin、YAP 和 PD-L1 的表达。进一步用慢病毒转染 H1299 和 A549 细胞，然后通过集落形成、CCK8、Transwell 和划痕愈合实验评估细胞增殖、迁移和侵袭。RT-qPCR 和 Western blot 用于定量分析 ezrin、PD-L1 和 YAP 的表达。此外，在体内评估 ezrin 在肿瘤生长中的作用，并通过免疫组织化学和 Western blot 评估小鼠样本中 ezrin 表达的变化。
NSCLC 中这些分子的阳性蛋白表达率如下：ezrin，43.9%（72/164）；YAP，54.3%（89/164）；PD-L1，47.6%（78/164）；均高于正常肺组织。此外，YAP 和 ezrin 的表达与 PD-L1 的表达呈正相关。Ezrin 促进了 NSCLC 中 YAP 和 PD-L1 的增殖、迁移、侵袭和表达。抑制 ezrin 表达可降低 ezrin 对细胞增殖、迁移、侵袭的影响，抑制 YAP 和 PD-L1 的表达，并明显减少体内实验肿瘤体积。
ezrin 在 NSCLC 患者中过度表达，并与 PD-L1 和 YAP 的表达相关。Ezrin 调节 YAP 和 PD-L1 的表达。抑制 ezrin 可延缓 NSCLC 的进展。
