黏着斑的体内磷酸化调节悖论

An in vivo phosphoregulation paradox for focal adhesions.

机构信息

Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute , National Institutes of Health, Bethesda, MD, USA.

出版信息

J Cell Biol. 2023 Mar 6;222(3). doi: 10.1083/jcb.202301060. Epub 2023 Feb 16.

DOI:10.1083/jcb.202301060

PMID:36795454

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9960130/

Abstract

Focal adhesions (FAs) dynamics regulate single cell migration. In this issue, Xue et al. (2023. J. Cell Biol.https://doi.org/10.1083/jcb.202206078) show that Y118 phosphorylation on Paxilin, a key FA protein, limits migration of cells in vivo. Unphosphorylated Paxilin is necessary for FA disassembly and cell motility. Their findings directly contradict results from in vitro experiments, emphasizing the need for recreating the in vivo complexity to understand how cells behave in their native environments.

摘要

焦点黏附（FAs）动力学调节单细胞迁移。在本期中，Xue 等人（2023.《细胞生物学杂志》https://doi.org/10.1083/jcb.202206078）表明，Paxilin 上的 Y118 磷酸化，这是一个关键的 FA 蛋白，限制了体内细胞的迁移。未磷酸化的 Paxilin 对于 FA 的解体和细胞运动是必需的。他们的发现直接与体外实验的结果相矛盾，强调需要重新创造体内的复杂性，以了解细胞在其天然环境中的行为。