Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China, Xiamen, PR China.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005721.
A novel marine bacterium designated strain PHK-P5 was isolated from a sea anemone ( sp. 1). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PHK-P5 belonged to the genus . The bacterium was Gram-stain-negative, aerobic, oxidase- and catalase- positive, oval- to rod-shaped, and motile. Growth was observed at pH 6.0-9.0, salinities of 2.0-9.0 % and temperatures of 4-37 °C. The G+C content of the chromosomal DNA was 49.2 %. The respiratory quinone was determined to be Q-10. The principal fatty acids of strain PHK-P5 were Ccyclo 8 (25.19 %), C (22.76 %), summed feature 8 (C 7/6; 16.14 %), C (8.81 %), Ccyclo (8.10 %), summed feature 2 (C aldehyde and/or unknown 10.928; 7.19 %) and C 7 11-methyl (5.03 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values among the genomes of strain PHK-P5 and the reference strains were 68.7-70.9 % and 17.4-18.1 %, respectively. The combined genotypic and phenotypic data showed that strain PHK-P5 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain PHK-P5 (=MCCC M21824=KCTC 82924).
一株新的海洋细菌菌株 PHK-P5 从海葵（sp.1）中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PHK-P5 属于 属。该细菌革兰氏染色阴性，需氧，氧化酶和过氧化氢酶阳性，呈椭圆形至杆状，运动。在 pH 值为 6.0-9.0、盐度为 2.0-9.0%和温度为 4-37°C 的条件下可生长。染色体 DNA 的 G+C 含量为 49.2%。菌株 PHK-P5 的主要呼吸醌为 Q-10。菌株 PHK-P5 的主要脂肪酸为 Ccyclo 8（25.19%）、C（22.76%）、summed feature 8（C 7/6；16.14%）、C（8.81%）、Ccyclo（8.10%）、summed feature 2（C 醛和/或未知 10.928；7.19%）和 C 7 11-甲基（5.03%）。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。菌株 PHK-P5 与参考菌株基因组之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 68.7-70.9%和 17.4-18.1%。综合表型和基因型数据表明，菌株 PHK-P5 代表 属中的一个新种，建议将其命名为 sp. nov.，其模式菌株为 PHK-P5（=MCCC M21824=KCTC 82924）。
