Liver folylpolyglutamate synthetase activity in folic acid-deficient rats.

To investigate the influence of folate deficiency on the addition process of glutamyl unit to folylpolyglutamates, liver folylpolyglutamate synthetase was studied. The enzyme activity appears slightly decreased in deficient liver as compared with control. This decline might be more evident in the hepatic cell owing to the lesser availability of reduced forms, preferred substrates of the enzyme.