Kahn H J, Baumal R
Am J Pathol. 1985 Jun;119(3):420-9.
Lectin binding to tumor cells in tissue sections of 16 nonmetastatic and 24 metastatic human adenocarcinomas and 5 nonmetastatic and 5 metastatic murine Lewis lung carcinomas (LLCs) was assessed with an avidin-biotin peroxidase technique. In human tumors, Ulex europaeus agglutinin I (UEA I) showed no binding; whereas concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), wheat germ agglutinin (WGA), soybean agglutinin (SBA), and Dolichos biflorus agglutinin (DBA) bound equally to primaries and metastases. However, peanut agglutinin (PNA) bound to less than 5% of cells in 37 of 40 primaries but to greater than 50% of cells in 18 of 24 metastases. In LLC tumors, UEA I and DBA showed no binding; whereas Con A, RCA I, and WGA bound equally to primaries and metastases. SBA bound to greater than 50% of cells in 5 metastases but not to the 5 primaries. There was less than 5% binding of PNA to 10 primary murine tumors after neuraminidase pretreatment of tissue sections but greater than 50% binding in 3 of 5 metastases. These studies indicate, in both human adenocarcinomas and an experimental tumor system, that most tumor cells which metastasize show preferential binding of PNA and SBA.
采用抗生物素蛋白-生物素过氧化物酶技术评估了凝集素与16例非转移性和24例转移性人腺癌以及5例非转移性和5例转移性小鼠Lewis肺癌（LLC）组织切片中肿瘤细胞的结合情况。在人类肿瘤中，欧洲荆豆凝集素I（UEA I）未显示结合；而伴刀豆球蛋白A（Con A）、蓖麻凝集素I（RCA I）、麦胚凝集素（WGA）、大豆凝集素（SBA）和双花扁豆凝集素（DBA）与原发肿瘤和转移瘤的结合情况相同。然而，花生凝集素（PNA）在40例原发肿瘤中的37例中与不到5%的细胞结合，但在24例转移瘤中的18例中与超过50%的细胞结合。在LLC肿瘤中，UEA I和DBA未显示结合；而Con A、RCA I和WGA与原发肿瘤和转移瘤的结合情况相同。SBA在5例转移瘤中与超过50%的细胞结合，但在5例原发肿瘤中未结合。在对组织切片进行神经氨酸酶预处理后，PNA与10例原发性小鼠肿瘤的结合率不到5%，但在5例转移瘤中的3例中结合率超过50%。这些研究表明，在人类腺癌和一个实验性肿瘤系统中，大多数发生转移的肿瘤细胞都显示出对PNA和SBA的优先结合。
