microRNA-128-3p 通过抑制 TUFT1 抑制胃癌细胞的增殖并加速其凋亡。

microRNA-128-3p inhibits proliferation and accelerates apoptosis of gastric cancer cells via inhibition of TUFT1.

机构信息

Department of Pathology, Yanan University Affiliated Hospital, Yan'an, 716000, Shaanxi, China.

Guangzhou Huayin Medical Laboratory Center. Ltd., Guangdong, 510000, Guangzhou, China.

出版信息

World J Surg Oncol. 2023 Feb 17;21(1):47. doi: 10.1186/s12957-023-02906-0.

OBJECTIVE

Gastric cancer (GC) is a malignant tumor rooting in the gastric mucosal epithelium, ranking the first among various malignant tumors. Therefore, the influence of microRNA-128-3p (miR-128-3p) by regulation of Tuftelin1 (TUFT1) on GC cells was investigated.

METHODS

The expression levels of miR-128-3p and TUFT1 in GC tissues and cells were detected. The correlation between miR-128-3p expression and overall survival of GC patients was analyzed. Human GC cells MGC803 were transfected with miR-128-3p or TUFT1-related oligonucleotides to figure their roles in viability, apoptosis, invasion, as well as epithelial-mesenchymal transition (EMT). The relationship between miR-128-3p and TUFT1 was validated.

RESULTS

miR-128-3p expression was low and TUFT1 expression was high in GC tissues. miR-128-3p expression was positively correlated with the overall survival of patients with GC. miR-128-3p targeted TUFT1. Up-regulated miR-128-3p or suppressed TUFT1 repressed viability, invasion, and EMT, and accelerated apoptosis of GC cells. Overexpressed TUFT1 reduced miR-128-3p-mediated growth inhibition of GC cells.

CONCLUSION

The study stresses that miR-128-3p can inhibit TUFT1 expression, thereby repressing GC cell activities.

目的

胃癌（GC）是一种起源于胃黏膜上皮的恶性肿瘤，在各种恶性肿瘤中排名第一。因此，研究了 microRNA-128-3p（miR-128-3p）通过 Tuftelin1（TUFT1）对 GC 细胞的影响。

方法

检测 GC 组织和细胞中 miR-128-3p 和 TUFT1 的表达水平。分析 miR-128-3p 表达与 GC 患者总生存率的相关性。用 miR-128-3p 或 TUFT1 相关寡核苷酸转染人 GC 细胞 MGC803，以研究其在细胞活力、凋亡、侵袭和上皮-间充质转化（EMT）中的作用。验证 miR-128-3p 和 TUFT1 之间的关系。

结果

GC 组织中 miR-128-3p 表达低，TUFT1 表达高。miR-128-3p 表达与 GC 患者的总生存率呈正相关。miR-128-3p 靶向 TUFT1。上调 miR-128-3p 或抑制 TUFT1 抑制 GC 细胞活力、侵袭和 EMT，并加速细胞凋亡。过表达 TUFT1 降低了 miR-128-3p 介导的 GC 细胞生长抑制作用。