Kang Wen, Shi Yuyuan, Yang Zhenlu, Yin Xindao, Zhao Ying, Weng Lixing, Teng Zhaogang
Department of Radiology, Nanjing First Hospital, Nanjing Medical University Nanjing 210006 P. R. China
College of Geography and Biological Information, Nanjing University of Posts and Telecommunications Nanjing 210023 P. R. China
RSC Adv. 2023 Feb 14;13(9):5609-5618. doi: 10.1039/d2ra06859a.
As a non-invasive cancer treatment, photodynamic therapy (PDT) has great applications in superficial tumors because of its high selectivity and low cumulative toxicity. However, the poor tumor-targeting ability and short blood circulation time of conventional photosensitizers (PSs) limit the efficacy of PDT to some extent. In this study, we synthesized flexible hollow human serum albumin (HHSA) and loaded photosensitizer Chlorin e6 (Ce6) and the chemotherapeutic drug Doxorubicin (DOX) for synergistic cancer therapy. HHSA can enhance drug delivery and cellular uptake through targeting gp60 and SPARC receptors and unique flexible hollow structures. The TEM images show that HHSA possesses distinct flexible hollow structures, as well as good monodispersity and deformability. After loading Ce6 and DOX, HHSA@Ce6-DOX displays better therapeutic effects than HHSA@DOX on the growth of 4T1 breast cancers without irradiation. Remarkably, it has a significantly higher therapeutic effect (relative cell activity: 45% 74%) than HHSA@Ce6 under 660 nm irradiation. Furthermore, the excellent biocompatibility of HHSA@Ce6-DOX has been proved both and , indicating that it has a promising future in synergistic tumor treatments.
作为一种非侵入性癌症治疗方法，光动力疗法（PDT）因其高选择性和低累积毒性而在浅表肿瘤中具有巨大的应用潜力。然而，传统光敏剂（PSs）较差的肿瘤靶向能力和较短的血液循环时间在一定程度上限制了PDT的疗效。在本研究中，我们合成了柔性中空人血清白蛋白（HHSA），并负载了光敏剂叶绿素e6（Ce6）和化疗药物阿霉素（DOX）用于协同癌症治疗。HHSA可以通过靶向gp60和SPARC受体以及独特的柔性中空结构来增强药物递送和细胞摄取。透射电子显微镜（TEM）图像显示HHSA具有独特的柔性中空结构，以及良好的单分散性和可变形性。负载Ce6和DOX后，HHSA@Ce6-DOX在未进行照射的情况下对4T1乳腺癌的生长显示出比HHSA@DOX更好的治疗效果。值得注意的是，在660 nm照射下，它比HHSA@Ce6具有显著更高的治疗效果（相对细胞活性：45% 74%）。此外，HHSA@Ce6-DOX的优异生物相容性在体内和体外均得到了证实，表明其在协同肿瘤治疗方面具有广阔的前景。
