Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.
Plant J. 2023 Jun;114(5):1149-1163. doi: 10.1111/tpj.16157. Epub 2023 Mar 3.
Plants are the most sophisticated biofactories and sources of food and biofuels present in nature. By engineering plant metabolism, the production of desired compounds can be increased and the nutritional or commercial value of the plant species can be improved. However, this can be challenging because of the complexity of the regulation of multiple genes and the involvement of different protein interactions. To improve metabolic engineering (ME) capabilities, different tools and strategies for rerouting the metabolic pathways have been developed, including genome editing and transcriptional regulation approaches. In addition, cutting-edge technologies have provided new methods for understanding uncharacterized biosynthetic pathways, protein degradation mechanisms, protein-protein interactions, or allosteric feedback, enabling the design of novel ME approaches.
植物是自然界中最复杂的生物工厂和食物及生物燃料的来源。通过对植物代谢进行工程设计，可以提高所需化合物的产量，并改善植物物种的营养价值或商业价值。然而，由于多个基因调控的复杂性以及不同蛋白质相互作用的参与，这可能具有挑战性。为了提高代谢工程（ME）的能力，已经开发了不同的工具和策略来重新路由代谢途径，包括基因组编辑和转录调控方法。此外，前沿技术为理解未被描述的生物合成途径、蛋白质降解机制、蛋白质-蛋白质相互作用或变构反馈提供了新的方法，从而能够设计新的 ME 方法。
