Rice Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Genetics and Breeding of High-Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of New Technology in Rice Breeding, Guangdong Rice Engineering Laboratory, Guangzhou, 510640, China.
Rice Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Genetics and Breeding of High-Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of New Technology in Rice Breeding, Guangdong Rice Engineering Laboratory, Guangzhou, 510640, China.
J Plant Physiol. 2023 Dec;291:154141. doi: 10.1016/j.jplph.2023.154141. Epub 2023 Nov 22.
Plant metabolism and development are a reflection of the orderly expression of genetic information intertwined with the environment interactions. Genome editing is the cornerstone for scientists to modify endogenous genes or introduce exogenous functional genes and metabolic pathways, holding immense potential applications in molecular breeding and biosynthesis. Over the course of nearly a decade of development, genome editing has advanced significantly beyond the simple cutting of double-stranded DNA, now enabling precise base and fragment replacements, regulation of gene expression and translation, as well as epigenetic modifications. However, the utilization of genome editing in plant synthetic metabolic engineering and developmental regulation remains exploratory. Here, we provide an introduction and a comprehensive overview of the editing attributes associated with various CRISPR/Cas tools, along with diverse strategies for the meticulous control of plant metabolic pathways and developments. Furthermore, we discuss the limitations of current approaches and future prospects for genome editing-driven plant breeding.
植物的新陈代谢和发育是遗传信息有序表达的反映，与环境相互作用交织在一起。基因组编辑是科学家修饰内源性基因或引入外源功能基因和代谢途径的基石，在分子育种和生物合成中具有巨大的潜在应用价值。在近十年的发展过程中，基因组编辑已经超越了简单的双链 DNA 切割，现在能够实现精确的碱基和片段替换、基因表达和翻译的调控以及表观遗传修饰。然而，基因组编辑在植物合成代谢工程和发育调控中的应用仍在探索之中。在这里，我们介绍并全面概述了各种 CRISPR/Cas 工具的编辑特性，以及精细控制植物代谢途径和发育的多种策略。此外，我们还讨论了当前方法的局限性和基于基因组编辑的植物育种的未来前景。
