Cotanche D A
Dept. of Anatomy and Cell Biology, Medical University of South Carolina, Charleston 29425.
Hear Res. 1987;30(2-3):197-206. doi: 10.1016/0378-5955(87)90136-5.
Damage to the tectorial membrane caused by acoustic trauma was examined with scanning and transmission electron microscopy immediately after exposure and at selected time points over a 10 day recovery period. At 0 h of recovery the structure of the tectorial membrane overlying the region of hair cell damage was severely disrupted and connections between the membrane and the basilar papilla were lost. By 24 h of recovery, regeneration of the tectorial membrane was evident in the secretion of new matrix materials by the supporting cells of the basilar papilla. By 10 days of recovery a new honeycomb-like matrix had replaced the segment of damaged tectorial membrane, re-established connections with hair cell stereocilia and become fused with adjacent regions of undamaged tectorial membrane. However, the regenerated segment included only the honeycomb-like structure of the lower layer of the normal tectorial membrane. The laterally-oriented fibers which form the upper layer of the membrane were not regenerated over the damaged region. These findings indicate that the tectorial membrane is regenerated in parallel with the hair cells during recovery from acoustic trauma but the full extent of this recovery and its effect on cochlear function are not yet clear.
在暴露后立即以及在10天恢复期间的选定时间点，用扫描电子显微镜和透射电子显微镜检查了声创伤引起的盖膜损伤。在恢复0小时时，覆盖毛细胞损伤区域的盖膜结构严重破坏，膜与基底乳头之间的连接丧失。到恢复24小时时，基底乳头支持细胞分泌新的基质材料，盖膜再生明显。到恢复10天时，一种新的蜂窝状基质取代了受损盖膜部分，与毛细胞静纤毛重新建立连接，并与未受损盖膜的相邻区域融合。然而，再生部分仅包括正常盖膜下层的蜂窝状结构。形成膜上层的横向纤维在受损区域未再生。这些发现表明，在声创伤恢复过程中，盖膜与毛细胞同时再生，但这种恢复的全部程度及其对耳蜗功能的影响尚不清楚。
