Zhao Ke-Xin, Zhang Ming-Yan, Yang Dan, Zhu Rong-Shu, Zhang Zi-Feng, Hu Ying-Hua, Kannan Kurunthachalam
International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), State Key Laboratory of Urban Water Resource and Environment/School of Environment, Harbin Institute of Technology (HIT), Harbin 150090, China.
Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150081, China.
J Hazard Mater. 2023 May 5;449:131002. doi: 10.1016/j.jhazmat.2023.131002. Epub 2023 Feb 13.
Human exposure to pesticides is a topic of public health concern for decades. Pesticide exposures have been assessed through the analysis of urine or blood matrices, but little is known on the accumulation of these chemicals in cerebrospinal fluid (CSF). CSF plays an important role in maintaining physical and chemical balance of the brain and central nervous system and any perturbation can have adverse effects on health. In this study, we investigated the occurrence of 222 pesticides in CSF from 91 individuals using gas chromatography-tandem mass spectrometry (GC-MS/MS). Measured pesticide concentrations in CSF were compared with those in 100 serum and urine specimens from individuals living in the same urban location. Twenty pesticides were found in CSF, serum and urine, at levels above the limit of detection. Three most frequently detected pesticides in CSF were biphenyl (100%), diphenylamine (75%), and hexachlorobenzene (63%). Median concentrations of biphenyl in CSF, serum and urine were 1.11, 10.6, and 1.10 ng/mL, respectively. Six triazole fungicides were found only in CSF, but not in other matrices. To our knowledge, this is the first study to report pesticide concentrations in CSF in a general urban population.
几十年来，人类接触农药一直是公共卫生关注的话题。农药接触情况已通过尿液或血液样本分析进行评估，但对于这些化学物质在脑脊液（CSF）中的蓄积情况却知之甚少。脑脊液在维持大脑和中枢神经系统的物理和化学平衡方面发挥着重要作用，任何干扰都可能对健康产生不利影响。在本研究中，我们使用气相色谱 - 串联质谱法（GC-MS/MS）调查了91名个体脑脊液中222种农药的存在情况。将脑脊液中测得的农药浓度与来自同一城市地区的100份血清和尿液样本中的浓度进行了比较。在脑脊液、血清和尿液中发现了20种农药，其含量高于检测限。脑脊液中最常检测到的三种农药是联苯（100%）、二苯胺（75%）和六氯苯（63%）。脑脊液、血清和尿液中联苯的中位浓度分别为1.11、10.6和1.10 ng/mL。六种三唑类杀菌剂仅在脑脊液中发现，在其他样本中未发现。据我们所知，这是第一项报道普通城市人群脑脊液中农药浓度的研究。
