嗜盐菌中的磷酸盐转运取决于细胞内的ATP水平。
Phosphate transport in Halobacterium halobium depends on cellular ATP levels.
作者信息
Zoratti M, Lanyi J K
机构信息
Consiglio Nazionale delle Ricerche Unit, University of Padova, Italy.
出版信息
J Bacteriol. 1987 Dec;169(12):5755-60. doi: 10.1128/jb.169.12.5755-5760.1987.
Abstract
Freshly harvested Halobacterium halobium cells grown in the presence of 0.5 mM Pi took up phosphate with a low apparent Km. Import depended on intracellular ATP levels; sodium and proton (electro)chemical gradients alone were not competent to drive Pi uptake. Although most of the phosphate accumulated as Pi in the cells, efflux of Pi was difficult to achieve.
摘要
在0.5 mM无机磷酸盐（Pi）存在的情况下培养的新鲜收获的嗜盐菌细胞，以较低的表观米氏常数（Km）摄取磷酸盐。摄取依赖于细胞内ATP水平；仅钠和质子（电）化学梯度不足以驱动磷酸盐摄取。尽管大部分磷酸盐在细胞内以Pi形式积累，但Pi的外流很难实现。
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本文引用的文献
1
Electrochemical proton gradient across the cell membrane of Halobacterium halobium: comparison of the light-induced increase with the increase of intracellular adenosine triphosphate under steady-state illumination.嗜盐菌细胞膜上的电化学质子梯度：稳态光照下光诱导增加与细胞内三磷酸腺苷增加的比较。
Biochemistry. 1980 Sep 30;19(20):4615-19. doi: 10.1021/bi00561a012.
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Electrochemical proton gradient across the cell membrane of Halobacterium halobium: effect of N,N'-dicyclohexylcarbodiimide, relation to intracellular adenosine triphosphate, adenosine diphosphate, and phosphate concentration, and influence of the potassium gradient.嗜盐菌细胞膜上的电化学质子梯度：N,N'-二环己基碳二亚胺的作用、与细胞内三磷酸腺苷、二磷酸腺苷及磷酸盐浓度的关系以及钾离子梯度的影响
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