Hartmann R, Sickinger H D, Oesterhelt D
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3821-5. doi: 10.1073/pnas.77.7.3821.
An energy-transducing pathway in halobacteria is described. Arginine mediates substrate level phosphorylation and allows the cells to grow anaerobically. Bacteriorhodopsin plus light can function as an alternative energy source under these conditions, provided the cells contain the pigment when transferred to the anaerobic environment. Therefore the selection of mutants functionally defective in ATP synthase or bacteriorhodopsin becomes possible.
描述了嗜盐菌中的一种能量转换途径。精氨酸介导底物水平磷酸化,并使细胞能够在厌氧条件下生长。在这些条件下,细菌视紫红质加光可作为替代能源,前提是细胞在转移到厌氧环境时含有这种色素。因此,选择在ATP合酶或细菌视紫红质功能上有缺陷的突变体成为可能。