大肠杆菌中钾离子转运与磷酸根离子转运之间联系的本质。
The nature of the link between potassium transport and phosphate transport in Escherichia coli.
作者信息
Russell L M, Rosenberg H
出版信息
Biochem J. 1980 Jun 15;188(3):715-23. doi: 10.1042/bj1880715.
Abstract
A series of mutants of Escherichia coli, combining defects in either of the two phosphate transport systems with defects in one or more of the potassium transport systems, was used to study the nature of the previously observed obligatory requirement for each one of these ions in the transport of the other. The results show that no pair of systems is obligatorily linked, and that either ion can be transported by any one of its systems, provided that a means of entry for the other ion is available. Furthermore, in the total absence of Pi, K+ entry accompanies the transport of other anions, such as aspartate, glutamate, sn-glycero-3-phosphate and glucose 6-phosphate. The results indicate that Pi and the other anions enter by symport with protons, and that a simultaneous K+/H+ exchange, which would serve to maintain the intracellular pH, is responsible for the observed K+ 'symport' with these anions.
摘要
利用一系列大肠杆菌突变体来研究先前观察到的现象，即在另一种离子的转运过程中，对这些离子中的每一种都存在强制性需求的本质。这些突变体在两种磷酸盐转运系统中的任何一种存在缺陷，同时在一种或多种钾转运系统中也存在缺陷。结果表明，没有哪一对系统是必然联系在一起的，并且只要有另一种离子的进入途径，任何一种离子都可以通过其自身的任何一个系统进行转运。此外，在完全没有无机磷酸盐（Pi）的情况下，钾离子（K+）的进入伴随着其他阴离子的转运，如天冬氨酸、谷氨酸、3-磷酸甘油和6-磷酸葡萄糖。结果表明，Pi和其他阴离子通过与质子协同转运进入细胞，并且观察到的这些阴离子与K+的“协同转运”是由同时发生的K+/H+交换所导致的，这种交换有助于维持细胞内的pH值。
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