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Establishing homologies in protein sequences.确定蛋白质序列中的同源性。
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New M13 vectors for cloning.用于克隆的新型M13载体。
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Penicillin acylase from E. coli: unique gene-protein relation.来自大肠杆菌的青霉素酰化酶：独特的基因-蛋白质关系。
Nucleic Acids Res. 1986 Jul 25;14(14):5713-27. doi: 10.1093/nar/14.14.5713.
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Molecular cloning and structure of the gene for 7 beta-(4-carboxybutanamido)cephalosporanic acid acylase from a Pseudomonas strain.来自假单胞菌菌株的7β-(4-羧基丁酰胺基)头孢菌素酸酰基转移酶基因的分子克隆与结构
J Bacteriol. 1985 Sep;163(3):1222-8. doi: 10.1128/jb.163.3.1222-1228.1985.
9
Structure of the penicillin acylase gene from Escherichia coli: a periplasmic enzyme that undergoes multiple proteolytic processing.来自大肠杆菌的青霉素酰化酶基因结构：一种经历多次蛋白水解加工的周质酶。
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10
Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.来自假单胞菌菌株的两种不同头孢菌素酰化酶基因的克隆与特性分析
J Bacteriol. 1987 Dec;169(12):5815-20. doi: 10.1128/jb.169.12.5815-5820.1987.

来自一株假单胞菌的两种不同头孢菌素酰基转移酶基因的核苷酸序列。

Nucleotide sequences of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.

作者信息

Matsuda A, Toma K, Komatsu K

机构信息

Pharmaceutical Research and Development Department, Asahi Chemical Industry Co., Ltd., Fuji, Japan.

出版信息

J Bacteriol. 1987 Dec;169(12):5821-6. doi: 10.1128/jb.169.12.5821-5826.1987.

DOI:10.1128/jb.169.12.5821-5826.1987
PMID:3680178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214163/
Abstract

Two genes, acyI and acyII, for distinct cephalosporin acylases from Pseudomonas sp. strain SE83 (A. Matsuda, K. Matsuyama, K. Yamamoto, S. Ichikawa, and K.I. Komatsu, J. Bacteriol. 169:5815-5820, 1987) were sequenced. Each sequence contained a single open reading frame for two nonidentical subunits, predicting a common precursor. Some homologies at the amino acid level were found between the acyII-encoded enzyme, but not the acyI-encoded one, and other related acylases.

摘要

对来自假单胞菌属菌株SE83（A. 松田、K. 松山、K. 山本、S. 市川和K.I. 小松，《细菌学杂志》169:5815 - 5820，1987年）的两种不同头孢菌素酰基转移酶的基因acyI和acyII进行了测序。每个序列都包含一个针对两个不同亚基的单一开放阅读框，预测有一个共同的前体。在acyII编码的酶（但不是acyI编码的酶）与其他相关酰基转移酶之间发现了一些氨基酸水平的同源性。