Matsuda A, Toma K, Komatsu K
Pharmaceutical Research and Development Department, Asahi Chemical Industry Co., Ltd., Fuji, Japan.
J Bacteriol. 1987 Dec;169(12):5821-6. doi: 10.1128/jb.169.12.5821-5826.1987.
Two genes, acyI and acyII, for distinct cephalosporin acylases from Pseudomonas sp. strain SE83 (A. Matsuda, K. Matsuyama, K. Yamamoto, S. Ichikawa, and K.I. Komatsu, J. Bacteriol. 169:5815-5820, 1987) were sequenced. Each sequence contained a single open reading frame for two nonidentical subunits, predicting a common precursor. Some homologies at the amino acid level were found between the acyII-encoded enzyme, but not the acyI-encoded one, and other related acylases.
对来自假单胞菌属菌株SE83(A. 松田、K. 松山、K. 山本、S. 市川和K.I. 小松,《细菌学杂志》169:5815 - 5820,1987年)的两种不同头孢菌素酰基转移酶的基因acyI和acyII进行了测序。每个序列都包含一个针对两个不同亚基的单一开放阅读框,预测有一个共同的前体。在acyII编码的酶(但不是acyI编码的酶)与其他相关酰基转移酶之间发现了一些氨基酸水平的同源性。