Tsuchiya K, Fukuyama S, Kanzaki N, Kanagawa K, Negoro S, Okada H
Department of Fermentation Technology, Osaka University, Japan.
J Bacteriol. 1989 Jun;171(6):3187-91. doi: 10.1128/jb.171.6.3187-3191.1989.
The nucleotide sequences of the genes for 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium sp. strain K172 (F-nylA) and Pseudomonas sp. NK87 (P-nylA), enzymes essential for the degradation of a by-product of the nylon-6 industry, were obtained by the dideoxynucleotide chain-termination method. A 1,479-base-pair open reading frame starting at a GTG and terminating at a TGA was found for the both of the genes. The P-nylA and F-nylA genes encoded polypeptides of 493 amino acids and had only 10 base substitutions in the coding region, which caused seven amino acid substitutions.
通过双脱氧核苷酸链终止法获得了黄杆菌属菌株K172（F-nylA）和假单胞菌属菌株NK87（P-nylA）的6-氨基己酸环二聚体水解酶基因的核苷酸序列，这两种酶是尼龙6工业副产物降解所必需的。发现这两个基因均有一个从GTG起始、TGA终止的1479个碱基对的开放阅读框。P-nylA和F-nylA基因编码493个氨基酸的多肽，编码区仅有10个碱基替换，导致7个氨基酸替换。
