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World J Microbiol Biotechnol. 1996 Jul;12(4):373-8. doi: 10.1007/BF00340214.
Aeromonas sp. ACY 95 produces constitutively and intracellularly a penicillin V acylase at an early stage of fermentation (12 h) and a cephalosporin C acylase at a later stage (36 h). Some penicillins, cephalosporin C and their side chain moieties/analogues, phenoxyacetic acid, penicillin V and penicillin G, enhanced penicillin V acylase production while none of the test compounds affected cephalosporin C acylase production. Supplementation of the medium with some sugars and sugar derivatives repressed enzyme production to varying degrees. The studies on enzyme formation, induction and repression, and substrate profile suggest that the cephalosporin C acylase and penicillin V acylase are two distinct enzymes. Substrate specificity studies indicate that the Aeromonas sp. ACY 95 produces a true cephalosporin C acylase which unlike the enzymes reported hitherto hydrolyses cephalosporin C specifically.
气单胞菌 ACY 95 在发酵早期（12 小时）持续地和细胞内产生青霉素 V 酰化酶，在后期（36 小时）产生头孢菌素 C 酰化酶。一些青霉素、头孢菌素 C 及其侧链部分/类似物、苯氧乙酸、青霉素 V 和青霉素 G 增强了青霉素 V 酰化酶的产生，而没有一种测试化合物影响头孢菌素 C 酰化酶的产生。培养基中添加一些糖和糖衍生物会不同程度地抑制酶的产生。对酶的形成、诱导和抑制以及底物谱的研究表明，头孢菌素 C 酰化酶和青霉素 V 酰化酶是两种不同的酶。底物特异性研究表明，气单胞菌 ACY 95 产生一种真正的头孢菌素 C 酰化酶，与迄今报道的酶不同，它特异性地水解头孢菌素 C。
