Ohya S, Yamazaki M, Sugawara S, Matsuhashi M
J Bacteriol. 1979 Jan;137(1):474-9. doi: 10.1128/jb.137.1.474-479.1979.
Penicillin-binding proteins in three species of Proteus, Proteus mirabilis, P. morganii, and P. rettgeri, were investigated by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. Penicillin-binding proteins in these Proteus species were compared with those in Escherichia coli K-12. An approximate correlation between penicillin-binding proteins in E. coli and those in Proteus species was shown by several criteria: electrophoretic mobilities; affinities of several beta-lactam antibiotics which show characteristic patterns of binding to penicillin-binding proteins in E. coli; relation between affinities of antibiotics to the proteins and effects on morphological changes in Proteus species; location of beta-lactamase activity among penicillin-binding proteins; and thermostability. The electrophoretic mobilities and several other characteristics of penicillin-binding proteins among the Proteus species examined were found to be similar from species to species and differed only slightly from those of E. coli.
通过十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳法，对奇异变形杆菌、摩根氏变形杆菌和雷氏变形杆菌这三种变形杆菌中的青霉素结合蛋白进行了研究。将这些变形杆菌中的青霉素结合蛋白与大肠杆菌K-12中的青霉素结合蛋白进行了比较。根据以下几个标准显示了大肠杆菌和变形杆菌属中青霉素结合蛋白之间的近似相关性：电泳迁移率；几种β-内酰胺抗生素的亲和力，这些抗生素对大肠杆菌中的青霉素结合蛋白显示出特征性的结合模式；抗生素与蛋白质的亲和力与对变形杆菌属形态变化的影响之间的关系；β-内酰胺酶活性在青霉素结合蛋白中的位置；以及热稳定性。在所检测的变形杆菌属中，青霉素结合蛋白的电泳迁移率和其他几个特征在不同菌种之间相似，与大肠杆菌的电泳迁移率和其他几个特征仅略有不同。
