Godfrey A J, Bryan L E
Antimicrob Agents Chemother. 1982 Feb;21(2):216-23. doi: 10.1128/AAC.21.2.216.
A mutant of Pseudomonas aeruginosa strain PAO503 was isolated after ethane-methane-sulfonate mutagenesis and selection of ticarcillin. The mutant, PCC17, displayed reduced affinity for [14C] penicillin G at all of its penicillin-binding proteins as well as a general increase in resistance to all the beta-lactam antibiotics tested. The mutation designated pbpA has been mapped by FP-2-mediated conjugation and was located distal to the proA locus and 33% linked to it. The two loci were not cotransducible with phage F116L. PCC17 and exconjugants produced from it had similar phenotypes, displayed the reduced affinity for [14C] penicillin G, had similar resistance profiles, and had an increased amount of protein corresponding to penicillin-binding protein 6. On back mutation the pbpA locus reverted to the PAO503 phenotype.
通过乙烷-甲磺酸诱变并筛选替卡西林后，分离出铜绿假单胞菌PAO503菌株的一个突变体。该突变体PCC17在其所有青霉素结合蛋白上对[14C]青霉素G的亲和力降低，并且对所有测试的β-内酰胺类抗生素的抗性普遍增加。命名为pbpA的突变已通过FP-2介导的接合进行定位，位于proA基因座的远端，与其有33%的连锁。这两个基因座不能被噬菌体F116L共转导。PCC17及其产生的接合子具有相似的表型，对[14C]青霉素G的亲和力降低，具有相似的抗性谱，并且与青霉素结合蛋白6相对应的蛋白量增加。pbpA基因座回复突变后恢复为PAO503表型。
