Iizuka R, Kaneko S, Aoki R, Kobayashi T
Department of Obstetrics and Gynaecology, School of Medicine, Keio University, Tokyo, Japan.
Hum Reprod. 1987 Oct;2(7):573-5. doi: 10.1093/oxfordjournals.humrep.a136591.
A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 +/- 1.8%) with a mean recovery of 23.3 +/- 6.4% (n = 6).
采用不连续的 Percoll 密度梯度法对携带人类 X 染色体的精子进行选择性分离。通过 Percoll 密度梯度的八个步骤沉降下来的富含携带 X 染色体精子的部分被授精给健康志愿者。六对夫妇成功怀孕，且每对夫妇均产下一名无异常的女婴。为了进一步分离，由 12 个步骤组成的 Percoll 密度梯度可使携带 X 染色体的精子纯化率达到 94%（被携带 Y 染色体精子的污染率为 6.4±1.8%），平均回收率为 23.3±6.4%（n = 6）。
