Department of Cardiology, The Affiliated Hospital of Chengde Medical University, Chengde, 067000, Hebei, China.
Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
Sci Rep. 2023 Feb 17;13(1):2831. doi: 10.1038/s41598-023-30035-x.
In this study, we explored the relationship between inflammatory adipokine levels and coronary artery disease (CAD). We collected subcutaneous adipose tissues(SAT), pericardial adipose tissues(PAT), and epicardial adipose tissues (EAT) and serum samples from 26 inpatients with CAD undergone coronary artery bypass grafting and 20 control inpatients without CAD. Serum inflammatory adipokines were measured by ELISA. Quantitative real-time PCR and western blot were used to measure gene and protein expression. Adipocyte morphology was assessed by H&E staining. Immunohistochemistry and immunofluorescence were used to measure endothelial and inflammatory markers. Serum pro- and anti-inflammatory adipokine levels were higher and lower, respectively, in the CAD group than those in the control group (P < 0.05). In CAD, the pro-inflammatory adipokine levels via ELISA in EAT and PAT were elevated. Pro-inflammatory adipokine mRNA expression was increased, while anti-inflammatory adipokine mRNA expression decreased, in CAD relative to NCAD in EAT and PAT rather than SAT. In EAT, adipocyte area and macrophage-specific staining were lower, while lymphatic vessel marker expression was higher in CAD. Additionally, the endothelial marker expression in EAT was higher than PAT in CAD. The three tissue types had different blood vessel amounts in CAD. The regulation and imbalance expression of the novel biomarkers, including inflammatory adipokine, macrophage infiltration, angiogenesis, and lymphangiogenesis in EAT and PAT, may be related to the pathogenesis of CAD. The serum levels of inflammatory adipokines may correlate to CAD, which requires large sample size studies to get further validation before clinic practice.
在这项研究中，我们探讨了炎症性脂肪因子水平与冠状动脉疾病（CAD）之间的关系。我们收集了 26 名接受冠状动脉旁路移植术的 CAD 住院患者和 20 名无 CAD 的对照住院患者的皮下脂肪组织（SAT）、心包脂肪组织（PAT）和心外膜脂肪组织（EAT）和血清样本。通过 ELISA 测量血清炎症性脂肪因子。使用定量实时 PCR 和 Western blot 测量基因和蛋白表达。通过 H&E 染色评估脂肪细胞形态。免疫组织化学和免疫荧光用于测量内皮和炎症标志物。与对照组相比，CAD 组血清促炎和抗炎脂肪因子水平分别升高和降低（P<0.05）。在 CAD 中，通过 ELISA 测定 EAT 和 PAT 中的促炎脂肪因子水平升高。与 NCAD 相比，EAT 和 PAT 中促炎脂肪因子的 mRNA 表达增加，而抗炎脂肪因子的 mRNA 表达降低。与对照组相比，EAT 中的脂肪细胞面积和巨噬细胞特异性染色减少，而淋巴管标记物的表达增加。此外，EAT 中的内皮标志物表达高于 PAT 中的 CAD。CAD 三种组织类型的血管数量不同。EAT 和 PAT 中炎症性脂肪因子、巨噬细胞浸润、血管生成和淋巴管生成的新生物标志物的调节和失衡表达可能与 CAD 的发病机制有关。炎症性脂肪因子的血清水平可能与 CAD 相关，需要更大的样本量研究进一步验证后才能在临床实践中应用。
