Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing Key Laboratory of Precision Medicine of Coronary Atherosclerotic Disease, Clinical Center for Coronary Heart Disease, Capital Medical University, Beijing 100029, China; Center of Geriatrics, Hainan General Hospital, Hainan 580000, China.
Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing Key Laboratory of Precision Medicine of Coronary Atherosclerotic Disease, Clinical Center for Coronary Heart Disease, Capital Medical University, Beijing 100029, China.
Life Sci. 2020 Mar 15;245:117338. doi: 10.1016/j.lfs.2020.117338. Epub 2020 Jan 22.
Secreted frizzled-related protein 5 (Sfrp5) primarily acts in combination with wingless-type family member 5a (Wnt5a), to inhibits chronic inflammation and repress atherosclerosis and other metabolic disorders. Epicardial adipose tissue (EAT), surrounding the heart and coronary arteries, has been found to be highly related to the progression of coronary artery disease through adipokines production. However, little is known about EAT-derived Sfrp5 and Wnt5a in humans. We aimed to investigate whether the EAT-derived Sfrp5/Wnt5a levels are altered in patients with CAD. Fifty-eight patients with CAD and 29 patients without CAD who underwent cardiac surgery were enrolled. Serum samples and paired adipose biopsies from EAT and subcutaneous adipose tissue (SAT) were collected, and Sfrp5 and Wnt5a levels were detected. Correlation and multivariate regression analyses were performed to determine the relationship between Sfrp5/Wnt5a expression and CAD and other clinical risk factors. According to the results, the CAD group had lower Sfrp5 and higher Wnt5a levels in EAT and serum (all p < 0.05). Serum Sfrp5 levels were significantly lower in CAD patients with impaired myocardial function. EAT Sfrp5 mRNA levels and serum Sfrp5 levels were both negatively associated with the presence of CAD, after adjustment for known biomarkers, EAT mRNA and serum Wnt5a levels correlated positively with the presence of CAD. Thus, we concluded that low Sfrp5 and high Wnt5a levels are associated with the presence of CAD, independent of other conventional risk factors.
分泌型卷曲相关蛋白 5（Sfrp5）主要与 Wnt5a 结合，以抑制慢性炎症并抑制动脉粥样硬化和其他代谢紊乱。已发现围绕心脏和冠状动脉的心脏外膜脂肪组织（EAT）通过产生脂肪因子与冠状动脉疾病的进展高度相关。然而，关于人类 EAT 衍生的 Sfrp5 和 Wnt5a 知之甚少。我们旨在研究 CAD 患者的 EAT 衍生的 Sfrp5/Wnt5a 水平是否发生改变。纳入 58 名 CAD 患者和 29 名接受心脏手术的无 CAD 患者。收集血清样本和来自 EAT 和皮下脂肪组织（SAT）的配对脂肪活检，并检测 Sfrp5 和 Wnt5a 水平。进行相关性和多元回归分析，以确定 Sfrp5/Wnt5a 表达与 CAD 和其他临床危险因素之间的关系。根据结果，EAT 和血清中的 CAD 组 Sfrp5 水平较低，Wnt5a 水平较高（均 p<0.05）。心肌功能受损的 CAD 患者血清 Sfrp5 水平明显降低。EAT Sfrp5 mRNA 水平和血清 Sfrp5 水平均与 CAD 的存在呈负相关，在校正已知生物标志物后，EAT mRNA 和血清 Wnt5a 水平与 CAD 的存在呈正相关。因此，我们得出结论，低 Sfrp5 和高 Wnt5a 水平与 CAD 的存在相关，与其他传统危险因素无关。
