利用 CRISPR/Cas9 技术生成端粒重复结合因子 1（TRF1）敲除的人类胚胎干细胞系 KRIBBe010-A-95、KRIBBe010-A-96 和 KRIBBe010-A-97。

Generation of telomeric repeat binding factor 1 (TRF1)-knockout human embryonic stem cell lines, KRIBBe010-A-95, KRIBBe010-A-96, and KRIBBe010-A-97, using CRISPR/Cas9 technology.

机构信息

Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Developmental and Reproductive Team, Korea Institute of Toxicology (KIT), Daejeon 34113, Republic of Korea.

Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.

出版信息

Stem Cell Res. 2023 Apr;68:103045. doi: 10.1016/j.scr.2023.103045. Epub 2023 Feb 13.

DOI:10.1016/j.scr.2023.103045

PMID:36805322

Abstract

Telomeric repeat binding factor 1 (TRF1) plays an essential role in maintaining telomere length. Here, we established TRF1-knockout human pluripotent stem cells (hPSCs; hTRF1-KO) using the CRISPR/Cas9 technology. The hTRF1-KO cell lines expressed pluripotency markers and demonstrated a normal karyotype (46, XX) and DNA profile. In addition, hTRF1-KOcells spontaneously differentiated into all three germ layers in vitro. Thus, these cell lines could be useful models in various research fields.

摘要

端粒重复结合因子 1（TRF1）在维持端粒长度方面发挥着重要作用。在这里，我们使用 CRISPR/Cas9 技术建立了 TRF1 敲除的人类多能干细胞（hPSCs；hTRF1-KO）。hTRF1-KO 细胞系表达多能性标记物，并表现出正常的核型（46,XX）和 DNA 图谱。此外，hTRF1-KO 细胞在体外自发分化为三个胚层。因此，这些细胞系可以成为各种研究领域的有用模型。