利用 CRISPR/Cas9 系统生成 S100B 纯合敲除多能干细胞系（WAe009-A-94）。
Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system.
机构信息
School of Stomatology, Lanzhou University, Lanzhou 730000, China.
State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Key Laboratory of Application of Pluripotent Stem Cells in Heart Regeneration, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
出版信息
Stem Cell Res. 2022 Oct;64:102924. doi: 10.1016/j.scr.2022.102924. Epub 2022 Sep 20.
S100 calcium binding protein beta (S100B) is an S-100 low molecular weight binding protein that regulates intracellular processes. This protein is involved in myocardial contractility and calcium handling capacity. In this study, a human embryonic stem cell (hESC) line with homozygous S100B knockout (S100B-KO) was generated using the CRISPR/Cas9 editing system. This S100B-KO hESC line maintained normal cell morphology and karyotype, expressed pluripotency markers, and could differentiate into cells of all three germ layers.
S100 钙结合蛋白 β（S100B）是一种 S-100 低分子量结合蛋白，可调节细胞内过程。这种蛋白质参与心肌收缩力和钙处理能力。在这项研究中，使用 CRISPR/Cas9 编辑系统生成了具有纯合 S100B 敲除（S100B-KO）的人胚胎干细胞（hESC）系。这种 S100B-KO hESC 系保持正常的细胞形态和核型，表达多能性标记物，并能分化为三个胚层的细胞。
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