利用 CRISPR/Cas9 技术生成 RNF1 缺陷型人多能干细胞系。
Generation of an RNF1-deficient human pluripotent stem cell line using CRISPR/Cas9 technology.
机构信息
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
出版信息
Stem Cell Res. 2022 Jul;62:102809. doi: 10.1016/j.scr.2022.102809. Epub 2022 May 8.
RNF1 (RING1A) is a catalytic component of the polycomb repressive complex 1 (PRC1) involved in regulation of, among others, embryonic development and disease progression. However, the exact role of RNF1 in self-renewal and differentiation of human embryonic stem cells (ESCs) remains unknown. Here, we derive one RNF1 knockout human ESC line using CRISPR/Cas9 system. The cell line retains the canonical stem cell morphology and normal karyotype. Moreover, the cell line highly expresses pluripotency genes and has three germ-layer differentiation potential. The RNF1 -/- cell line will be useful for studies on the function and role of RNF1 in human embryonic stem cell fate decisions.
RNF1（RING1A）是多梳抑制复合物 1（PRC1）的催化亚基，参与胚胎发育和疾病进展等多种调控。然而，RNF1 在人类胚胎干细胞（ESC）自我更新和分化中的确切作用仍不清楚。在这里，我们使用 CRISPR/Cas9 系统获得了一个 RNF1 敲除的人类 ESC 系。该细胞系保持了典型的干细胞形态和正常核型。此外，该细胞系高表达多能性基因，并具有三胚层分化潜能。RNF1 -/-细胞系将有助于研究 RNF1 在人类胚胎干细胞命运决定中的功能和作用。
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