Zhang Feifei, Zhang Kan, Zhang Zhiqian, Chen Hai-Qi, Chen Xiao-Wei, Xian Xing-You, Wu Yi-Rui
Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, China.
Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, China.
Bioresour Technol. 2023 Apr;373:128751. doi: 10.1016/j.biortech.2023.128751. Epub 2023 Feb 15.
To elevate the efficiency of acetone-butanol-ethanol (ABE) fermentation by the wild-type strain WK, an optimal co-utilization system (20 mM Fe and 5 g/L butyrate) was established to bring about a 22.22% increment in the yield of ABE mixtures with a significantly enhanced productivity (0.32 g/L/h). With the heterologous introduction of the secondary alcohol dehydrogenase encoded gene (adh), more than 95% of acetone was eliminated to convert 4.5 g/L isopropanol with corresponding increased butanol and ethanol production by 21.08% and 65.45% in the modified strain WK::adh. Under the optimal condition, strain WK::adh was capable of producing a total of 25.46 g/L IBE biosolvents with an enhanced productivity of 0.35 g/L/h by 45.83% over the original conditions. This work for the first time successfully established a synergetic system of co-utilizing Fe(III) and butyrate to demonstrate a feasible and efficient manner for generating the value-added biofuels through the metabolically engineered solventogenic clostridial strain.
为提高野生型菌株WK的丙酮-丁醇-乙醇（ABE）发酵效率，建立了一种最佳共利用系统（20 mM铁和5 g/L丁酸盐），使ABE混合物产量提高了22.22%，生产率显著提高（0.32 g/L/h）。通过异源引入仲醇脱氢酶编码基因（adh），在改造后的菌株WK::adh中，超过95%的丙酮被消除，转化为4.5 g/L异丙醇，同时丁醇和乙醇产量相应提高了21.08%和65.45%。在最佳条件下，菌株WK::adh能够总共生产25.46 g/L IBE生物溶剂，生产率提高到0.35 g/L/h，比原始条件提高了45.83%。这项工作首次成功建立了一种协同利用铁（III）和丁酸盐的系统，以展示通过代谢工程改造的产溶剂梭菌菌株生产增值生物燃料的可行且高效的方式。
