Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, 06070, Ankara, Turkey.
Department of Medical Biology, Faculty of Medicine, Sakarya University, 54050, Sakarya, Turkey.
Chem Biodivers. 2023 Mar;20(3):e202201263. doi: 10.1002/cbdv.202201263. Epub 2023 Mar 6.
Honeybees provide multiple products such as bee venom (BV) which are used for various nutritional and medicinal purposes. BV has received great attention due to its wide range of bioactive components with potential anti-cancer effects on different cancers. Triple negative breast cancer (TNBC) is defined as an aggressive type of breast cancer and new therapeutic targets are required for its treatment. In the current literature information is varied about the composition and quantity of BV bioactive compounds as well as the origin of BV and its significance. In this context, the cytotoxic and apoptotic effects of BV with a higher rate of mellitin from Apis mellifera anatoliaca (Muğla ecotype) on MDA-MB-231 cells was evaluated, in vitro. The cytotoxic, apoptotic and morphological effects of BV were determined by WST-1, Annexin V, cell cycle analysis and Acridine Orange staining. The results showed that BV caused apoptotic cell death in TNBC cells at a lower dose (0.47 μg/mL, p<0.01). This study suggests that BV could be developed as a potential therapeutic agent for cancer treatment. However, the mechanism of BV-induced apoptosis death should be clarified at the molecular level.
蜜蜂提供多种产品，如蜂毒 (BV)，可用于各种营养和药用目的。BV 因其具有广泛的生物活性成分而备受关注，对不同癌症具有潜在的抗癌作用。三阴性乳腺癌 (TNBC) 被定义为一种侵袭性乳腺癌，需要新的治疗靶点。在当前的文献中，关于 BV 生物活性化合物的组成和数量以及 BV 的来源及其意义存在差异。在这种情况下，评估了来自 Apis mellifera anatoliaca (Muğla 生态型) 的 mellitin 比率更高的 BV 对 MDA-MB-231 细胞的体外细胞毒性和凋亡作用。通过 WST-1、Annexin V、细胞周期分析和吖啶橙染色来确定 BV 的细胞毒性、凋亡和形态学作用。结果表明，BV 在较低剂量（0.47μg/mL，p<0.01）下导致 TNBC 细胞发生凋亡性细胞死亡。这项研究表明，BV 可以开发为癌症治疗的潜在治疗剂。然而，应在分子水平上阐明 BV 诱导凋亡死亡的机制。
