Miller D J, Hunter A G
Department of Animal Science, University of Minnesota, St. Paul 55108.
J Dairy Sci. 1987 Oct;70(10):2150-3. doi: 10.3168/jds.S0022-0302(87)80267-9.
Individual semen samples from 29 bulls in routine artificial breeding service were tested for their capability to achieve in vitro fertilization. Ejaculated semen was diluted, .1 ml of semen in 2 ml of a modified Tyrode's medium with an osmolality of 340 mOsmol/kg, washed thrice, incubated 3 h at 37 degrees C before being used for in vitro fertilization, or incubated 4 h and 8 h before assessment of motility, capacitation, and acrosome integrity. The degree of variability in percentage of oocytes fertilized was assessed along with several factors that might contribute to this variation. Variation among bulls was not significantly different. Variation from one replicate to another was high. Variation was found in motility, capacitation, and frequency of acrosome reaction, but these variables were not significantly correlated to fertilization rate in vitro.
对29头常规人工授精服务中的公牛的个体精液样本进行了体外受精能力测试。采集的精液进行稀释，将0.1毫升精液加入2毫升渗透压为340毫摩尔/千克的改良Tyrode's培养基中，洗涤三次，在37℃下孵育3小时后用于体外受精，或在评估活力、获能和顶体完整性之前分别孵育4小时和8小时。评估了受精卵母细胞百分比的变异程度以及可能导致这种变异的几个因素。公牛之间的变异没有显著差异。不同重复之间的变异很大。在活力、获能和顶体反应频率方面发现了变异，但这些变量与体外受精率没有显著相关性。
