Gambert P, Lallemant C, Archambault A, Maume B F, Padieu P
J Chromatogr. 1979 Jan 1;162(1):1-6. doi: 10.1016/s0378-4347(00)82057-5.
A gas-liquid chromatographic (GLC) method and an isotopic dilution-mass fragmentographic (ID-MF) procedure using the same capillary chromatographic separation are described for serum cholesterol assay. GLC included silylation and separation on a highly efficient glass capillary column which allowed the separation of cholesterol from cholestanol and the use of epicoprostanol as internal standard. The concentrations were calculated from the areas of the signals and digitalized by a reporting integrator. The reproducibility was 0.5% and the correlation with the ID-MF technique was 0.997. The ID-MF technique was characterized by the use of [3,4-13C] cholesterol as the labelled standard and a chemical ionization mode. The reproducibility was 0.8%.
本文描述了一种用于血清胆固醇测定的气液色谱（GLC）方法和一种使用相同毛细管色谱分离的同位素稀释-质谱碎片分析法（ID-MF）。GLC包括硅烷化和在高效玻璃毛细管柱上的分离，该柱可将胆固醇与胆甾烷醇分离，并使用表普罗布考作为内标。浓度根据信号面积计算，并由报告积分仪数字化。重现性为0.5%，与ID-MF技术的相关性为0.997。ID-MF技术的特点是使用[3,4-13C]胆固醇作为标记标准物和化学电离模式。重现性为0.8%。
