State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P. R. China.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P. R. China.
Environ Sci Technol. 2023 Mar 7;57(9):3980-3989. doi: 10.1021/acs.est.2c07182. Epub 2023 Feb 21.
Nanopesticides are considered to be a promising alternative strategy for enhancing bioactivity and delaying the development of pathogen resistance to pesticides. Here, a new type of nanosilica fungicide was proposed and demonstrated to control late blight by inducing intracellular peroxidation damage to , the pathogen associated with potato late blight. Results indicated that the structural features of different silica nanoparticles were largely responsible for their antimicrobial activities. Mesoporous silica nanoparticles (MSNs) exhibited the highest antimicrobial activity with a 98.02% inhibition rate of , causing oxidative stress responses and cell structure damage in . For the first time, MSNs were found to selectively induce spontaneous excess production of intracellular reactive oxygen species in pathogenic cells, including hydroxyl radicals (•OH), superoxide radicals (•O), and singlet oxygen (O), leading to peroxidation damage in . The effectiveness of MSNs was further tested in the pot experiments as well as leaf and tuber infection, and successful control of potato late blight was achieved with high plant compatibility and safety. This work provides new insights into the antimicrobial mechanism of nanosilica and highlights the use of nanoparticles for controlling late blight with green and highly efficient nanofungicides.
纳米农药被认为是一种有前途的替代策略，可以提高生物活性并延缓病原体对农药产生抗药性。在这里，提出了一种新型纳米硅杀菌剂，通过诱导与马铃薯晚疫病相关的病原体的细胞内过氧化损伤来控制晚疫病。结果表明，不同硅纳米颗粒的结构特征在很大程度上决定了它们的抗菌活性。介孔硅纳米颗粒（MSNs）表现出最高的抗菌活性，对 的抑制率达到 98.02%，导致 中的氧化应激反应和细胞结构损伤。MSNs 首次被发现能够选择性地诱导病原细胞内活性氧（ROS）的自发过量产生，包括羟基自由基（•OH）、超氧自由基（•O）和单线态氧（O），导致 在过氧化损伤。MSNs 的有效性在盆栽实验以及叶片和块茎感染中得到了进一步测试，并成功地控制了马铃薯晚疫病，具有高植物相容性和安全性。这项工作为纳米硅的抗菌机制提供了新的见解，并强调了使用纳米颗粒作为绿色、高效的纳米杀菌剂来控制晚疫病。
