Kubín M, Svandová E, Stastná J
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
J Hyg Epidemiol Microbiol Immunol. 1987;31(3):327-33.
Intradermal skin tests with a 2TU dose of PPD-RT 23 prepared from M. tuberculosis and 0.1 ug/0.1 ml of PPD-RS 631 from M. xenopi were simultaneously carried out in 378 7-year-old children from two localities in North-Bohemian region's capital Ustí n. Lab., a focus of M. xenopi endemicity repeatedly confirmed since its disclosure in 1980 by positive M. xenopi isolations from humans and public water supply network. A further group 157 children serving as controls was from Prague district 4 where no presence of M. xenopi strains was ever recorded. All of these children had received routine immunization at birth with Czech BCG vaccine. The children from the two endemic localities were found to give a positive 6 mm or greater reaction to M. xenopi mycobacterin in 43.3% and 22.3%, to human tuberculin in 12.8% and 12.6%, respectively. The frequency histogram clearly separated a group of reactors with 8-18 mm indurations from a group of nonreactors showing a skin induration of 4-8 mm. The higher reactivity of this exposed child population was also reflected in a larger proportion of reactions greater to M. xenopi PPD than to human tuberculin antigen: the reactions greater by 1-5 mm accounted, respectively, for 25.1% and 20.6%, reactions greater by 6 mm or more for 23.7% and 15.9%. Among a group of children from Prague district 4, 6.4% had medium-sized and 3.8% large-sized reactions to M. xenopi antigen; the proportion of reactions greater to M. xenopi antigen than to human tuberculin accounted for only 5.1%, reactions greater to tuberculin than to sensitin were here in slight predominance. The evidenced skin sensitization to M. xenopi mycobacterin is suggested to result from the different degrees of exposure to infection by environmental mycobacteria.
对来自北波希米亚地区首府乌斯季-纳拉博尔姆两个地方的378名7岁儿童同时进行了皮内皮肤试验，分别用从结核分枝杆菌制备的2TU剂量的PPD-RT 23和来自蟾分枝杆菌的0.1μg/0.1ml的PPD-RS 631进行试验。乌斯季-纳拉博尔姆自1980年发现以来，多次从人类和公共供水网络中分离出蟾分枝杆菌阳性菌株，是蟾分枝杆菌的一个地方性流行区。另一组157名儿童作为对照，来自布拉格第4区，那里从未记录到蟾分枝杆菌菌株的存在。所有这些儿童出生时都接种了捷克卡介苗。发现来自两个地方性流行区的儿童中，对蟾分枝杆菌菌素产生6mm或更大阳性反应的分别占43.3%和22.3%，对人结核菌素产生阳性反应的分别占12.8%和12.6%。频率直方图清楚地将硬结为8-18mm的反应者组与硬结为4-8mm的无反应者组区分开来。这种暴露儿童群体较高的反应性还体现在对蟾分枝杆菌PPD的反应大于对人结核菌素抗原的反应的比例更大：反应大1-5mm的分别占25.1%和20.6%，反应大6mm或更大的分别占23.7%和15.9%。在来自布拉格第4区的一组儿童中，6.4%对蟾分枝杆菌抗原有中等大小的反应，3.8%有大的反应；对蟾分枝杆菌抗原反应大于对人结核菌素反应的比例仅占5.1%，对结核菌素反应大于对变应原的反应在此略占优势。所证明的对蟾分枝杆菌菌素的皮肤致敏作用被认为是由于环境分枝杆菌感染的不同暴露程度所致。
