马拉维北部对环境和结核分枝杆菌抗原自然获得性免疫反应的模式及影响

Patterns and implications of naturally acquired immune responses to environmental and tuberculous mycobacterial antigens in northern Malawi.

作者信息

Black G F, Dockrell H M, Crampin A C, Floyd S, Weir R E, Bliss L, Sichali L, Mwaungulu L, Kanyongoloka H, Ngwira B, Warndorff D K, Fine P E

机构信息

Karonga Prevention Study, Chilumba, Malawi.

出版信息

J Infect Dis. 2001 Aug 1;184(3):322-9. doi: 10.1086/322042. Epub 2001 Jul 10.

DOI:10.1086/322042

PMID:11443558

Abstract

Interferon (IFN)-gamma responsiveness to 12 purified protein derivative (PPD) and new tuberculin antigens from 9 species of mycobacteria was assessed, using a whole blood assay, in 616 young adults living in northern Malawi, where Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccination provides no protection against pulmonary tuberculosis. The prevalence of IFN-gamma responsiveness was highest for PPDs of M. avium, M. intracellulare, and M. scrofulaceum (the MAIS complex). Correlations between responsiveness paralleled genetic relatedness of the mycobacterial species. A randomized, controlled trial was carried out, to assess the increase in IFN-gamma responsiveness to M. tuberculosis PPD that can be attributed to M. bovis BCG vaccination. The BCG-attributable increase in IFN-gamma response to M. tuberculosis PPD was greater for individuals with low initial responsiveness to MAIS antigens than for those with high initial responsiveness. Although not statistically significant, the trend is consistent with the hypothesis that prior exposure to environmental mycobacteria interferes with immune responses to BCG vaccination.

摘要

在马拉维北部生活的616名年轻成年人中，采用全血检测法评估了γ干扰素对12种纯化蛋白衍生物（PPD）以及来自9种分枝杆菌的新型结核菌素抗原的反应性。在该地，卡介苗（BCG）接种不能预防肺结核。γ干扰素反应性的患病率在鸟分枝杆菌、胞内分枝杆菌和瘰疬分枝杆菌（MAIS复合体）的PPD中最高。反应性之间的相关性与分枝杆菌物种的遗传相关性平行。开展了一项随机对照试验，以评估可归因于卡介苗接种的γ干扰素对结核分枝杆菌PPD反应性的增加情况。对于初始对MAIS抗原反应性低的个体，卡介苗接种导致的γ干扰素对结核分枝杆菌PPD反应性的增加幅度大于初始反应性高的个体。虽然无统计学显著性，但该趋势与以下假设一致，即先前暴露于环境分枝杆菌会干扰对卡介苗接种的免疫反应。