Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, N13W7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan.
Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, N13W7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan.
J Oral Biosci. 2023 Jun;65(2):202-205. doi: 10.1016/j.job.2023.02.002. Epub 2023 Feb 19.
Graphene oxide (GO) is a nanocarbon material with a high aspect ratio (width:thickness) and abundant anionic functional groups on its surface. In this study, we attached GO to the surface of medical gauze fibers, constructed a complex with a cationic surface active agent (CSAA), and demonstrated that the treated gauze exhibits antibacterial activity even after rinsing with water.
Medical gauze was immersed in GO dispersion (0.001%, 0.01%, and 0.1%), rinsed with water, dried, and subjected to the Raman spectroscopy analysis. Subsequently, the gauze treated with 0.001% GO dispersion was immersed in 0.1% cetylpyridinium chloride (CPC) solution, immediately rinsed with water, and dried. Untreated, GO-only, and CPC-only gauzes were prepared for comparison. Each gauze was placed in a culture well, seeded with Escherichia coli or Actinomyces naeslundii, and turbidity was measured after 24 h of incubation.
The Raman spectroscopy analysis of the gauze after immersion and rinsing showed a G band peak, indicating that GO remained on the surface of the gauze. The turbidity measurements indicated that GO/CPC-treated gauze (GO-treated and rinsed, followed by CPC-treatment and rinsing) significantly decreased turbidity compared to the other gauzes (P < 0.05), suggesting that the GO/CPC complex remained on the gauze fibers even after water rinsing and showed antibacterial activity.
The GO/CPC complex imparts water-resistant antibacterial properties to gauze and has the potential to be widely used for the antimicrobial treatment of clothes.
氧化石墨烯（GO）是一种具有高纵横比（宽：厚）和丰富阴离子官能团的纳米碳材料。在本研究中，我们将 GO 附着在医用纱布纤维表面，构建了与阳离子表面活性剂（CSAA）的复合物，并证明即使用水冲洗后，处理过的纱布仍具有抗菌活性。
将医用纱布浸入 GO 分散液（0.001%、0.01%和 0.1%）中，用清水冲洗，干燥，并进行拉曼光谱分析。随后，将用 0.001%GO 分散液处理的纱布浸入 0.1%十六烷基氯化吡啶（CPC）溶液中，立即用清水冲洗并干燥。制备未处理、仅含 GO 和仅含 CPC 的纱布作为对照。将每个纱布放入培养皿中，接种大肠杆菌或奈瑟氏放线菌，孵育 24 小时后测量浊度。
纱布浸泡和冲洗后的拉曼光谱分析显示出 G 带峰，表明 GO 仍留在纱布表面。浊度测量表明，GO/CPC 处理的纱布（GO 处理和冲洗，然后 CPC 处理和冲洗）与其他纱布相比，浊度显著降低（P<0.05），表明 GO/CPC 复合物即使在水冲洗后仍留在纱布纤维上，并具有抗菌活性。
GO/CPC 复合物赋予纱布耐水抗菌性能，并有可能广泛用于衣物的抗菌处理。
