Estrela Carlos, Sousa-Neto Manoel Damião, Alves Denise Ramos Silveira, Alencar Ana Helena Gonçalves, Santos Tatiane Oliveira, Pécora Jesus Djalma
Dental School, UFG - Federal University of Goiás, Goiânia, GO, Brazil.
Braz Dent J. 2012;23(6):645-53. doi: 10.1590/s0103-64402012000600004.
The aim of this preliminary study was to verify the antibacterial potential of cetylpyridinium chloride (CPC) in root canals infected by Enterococcus faecalis. Forty human maxillary anterior teeth were prepared and inoculated with E. faecalis for 60 days. The teeth were randomly assigned to the following groups: 1: Root canal preparation (RCP) + 0.1% CPC with positive-pressure irrigation (PPI, Conventional, NaviTip(®)); 2: RCP + 0.2% CPC PPI; 3: RCP + 2.5% NaOCl PPI; 4: RCP + 2.5% NaOCl with negative-pressure irrigation system (NPI, EndoVac(®)); 5: Positive control; and 6: Negative control. Four teeth of each experimental group were evaluated by culture and 4 by scanning electron microscopy (SEM). In all teeth, the root canals were dried and filled with 17% EDTA (pH 7.2) for 3 min for smear layer removal. Samples from the infected root canals were collected and immersed in 7 mL of Letheen Broth (LB), followed by incubation at 37°C for 48 h. Bacterial growth was analyzed by turbidity of culture medium and then observed with a UV spectrophotometer. The irrigating solutions were further evaluated for antimicrobial effect by an agar diffusion test.The statistical data were treated by means, standard deviation, Kruskal-Wallis test and analysis of variance. Significance level was set at 5%. The results showed the presence of E. faecalis after root canal sanitization. The number of bacteria decreased after the use of CPC. In the agar diffusion test, CPC induced large microbial inhibition zones, similar to 2% chlorhexidine and large than 2.5% NaOCl. In conclusion, cetylpyridinium chloride showed antibacterial potential in endodontic infection with E. faecalis.
这项初步研究的目的是验证十六烷基氯化吡啶（CPC）对受粪肠球菌感染的根管的抗菌潜力。制备了40颗人上颌前牙，接种粪肠球菌60天。这些牙齿被随机分为以下几组：1：根管预备（RCP）+0.1%CPC正压冲洗（PPI，传统方法，NaviTip®）；2：RCP+0.2%CPC PPI；3：RCP+2.5%次氯酸钠PPI；4：RCP+2.5%次氯酸钠负压冲洗系统（NPI，EndoVac®）；5：阳性对照；6：阴性对照。每个实验组的4颗牙齿通过培养进行评估，4颗通过扫描电子显微镜（SEM）进行评估。在所有牙齿中，根管干燥后用17%乙二胺四乙酸（pH 7.2）填充3分钟以去除玷污层。从感染的根管中收集样本并浸入7毫升Letheen肉汤（LB）中，然后在37°C下孵育48小时。通过培养基的浊度分析细菌生长情况，然后用紫外分光光度计进行观察。通过琼脂扩散试验进一步评估冲洗液的抗菌效果。统计数据采用均值、标准差、Kruskal-Wallis检验和方差分析进行处理。显著性水平设定为5%。结果显示根管消毒后存在粪肠球菌。使用CPC后细菌数量减少。在琼脂扩散试验中，CPC诱导出较大的微生物抑制圈，类似于2%洗必泰且大于2.5%次氯酸钠。总之，十六烷基氯化吡啶在粪肠球菌引起的牙髓感染中显示出抗菌潜力。
