Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
Department of Chemistry for Life Sciences and Agriculture, Tokyo University of Agriculture, Tokyo, Japan.
Biosci Biotechnol Biochem. 2023 Apr 24;87(5):563-568. doi: 10.1093/bbb/zbad012.
Terpenoid is an important group of compounds not only as biocomponents but also as useful secondary metabolites. A volatile terpenoid 1,8-cineole, which is used as a food additive, flavoring agent, cosmetic, etc., is also attracting attention from a medical perspective due to its antiinflammation and antioxidation. The 1,8-cineole fermentation using a recombinant Escherichia coli strain has been reported, although a carbon source supplement is necessary for a high-yield 1,8-cineole production. We constructed the 1,8-cineole-producing cyanobacteria toward a carbon-free and sustainable 1,8-cineole production. cnsA, the 1,8-cineole synthase gene in Streptomyces clavuligerus ATCC 27064, was introduced and overexpressed in the cyanobacterium Synechococcus elongatus PCC 7942. We succeeded in producing an average of 105.6 µg g-1 wet cell weight of 1,8-cineole in S. elongatus 7942 without supplementing any carbon source. Using the cyanobacteria expression system is an efficient approach to producing 1,8-cineole by photosynthesis.
萜类化合物不仅是生物成分的重要组成部分，也是有用的次生代谢物。一种挥发性萜类化合物 1,8-桉叶油醇，可用作食品添加剂、香料、化妆品等，由于其具有抗炎和抗氧化作用，也引起了医学界的关注。已经有报道称使用重组大肠杆菌菌株发酵 1,8-桉叶油醇，但要高产 1,8-桉叶油醇，需要补充碳源。我们构建了能够生产 1,8-桉叶油醇的蓝藻，以实现无碳和可持续的 1,8-桉叶油醇生产。我们将链霉菌属棒状链霉菌 ATCC 27064 中的 1,8-桉叶油醇合酶基因 cnsA 导入并在蓝藻集胞藻 PCC 7942 中过表达。我们成功地在 S.elongatus 7942 中生产了平均 105.6μg g-1 湿细胞重量的 1,8-桉叶油醇，而无需补充任何碳源。利用蓝藻表达系统通过光合作用生产 1,8-桉叶油醇是一种有效的方法。
