Kanno H
Department of Orthopedic Surgery, Akita University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1987 Jun;61(6):755-64.
Catalase activity of rheumatoid synovial fluid measured by a gasometry was higher than that of osteoarthritic fluids, suggesting a possible origin of the catalase activity from synovial fluid leukocytes. Isoelectric focusing polyacrylamide gel electrophoresis of the extract from polymorphonuclear leukocytes (PMN) of rheumatoid synovial fluid exhibited two to four achromatic bands and one dark band of catalase activity. Catalase staining of synovial fluid cells with diaminobenzidine revealed a marked activity in PMN, no or weak activity in monocytes and negative activity in lymphocytes. Catalase scores of the PMN in rheumatoid synovial fluid were lower than those of osteoarthritic fluid and of normal peripheral blood PMN. Ultrastructural localization of catalase activity in leukocytes of rheumatoid synovial fluid was examined by diaminobenzidine staining. Electron dense deposits were observed in granules, probably peroxisomes and in cytoplasm of the PMN and also in microperoxisomes of the monocyte.
通过气体分析法测定的类风湿性滑液过氧化氢酶活性高于骨关节炎滑液，提示过氧化氢酶活性可能来源于滑液白细胞。类风湿性滑液多形核白细胞（PMN）提取物的等电聚焦聚丙烯酰胺凝胶电泳显示出两到四条无色带和一条过氧化氢酶活性暗带。用二氨基联苯胺对滑液细胞进行过氧化氢酶染色，结果显示PMN中有明显活性，单核细胞中无或活性较弱，淋巴细胞中无活性。类风湿性滑液中PMN的过氧化氢酶评分低于骨关节炎滑液和正常外周血PMN。通过二氨基联苯胺染色检查了类风湿性滑液白细胞中过氧化氢酶活性的超微结构定位。在颗粒中观察到电子致密沉积物，可能是过氧化物酶体，存在于PMN的细胞质中，也存在于单核细胞的微过氧化物酶体中。
