Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, China.
Jiangsu Province Engineering Research Center of Eco-cultivation and High-value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, China.
Pest Manag Sci. 2023 Jul;79(7):2423-2432. doi: 10.1002/ps.7420. Epub 2023 Mar 10.
Rice blast caused by Magnaporthe oryzae is one of the most devastating diseases of rice, and novel fungicides for controlling rice blast are needed owing to the problem of resistance to commonly used control agents. We previously found that methanol extract of Lycoris radiata (L'Her.) Herb. showed an excellent inhibitory effect on mycelial growth of M. oryzae, indicating its potential for developing control agents against M. oryzae. In this study, we aim to investigate the antifungal effects of different Lycoris spp. against M. oryzae, and clarify the main active components.
Extracts from bulbs of seven Lycoris spp. showed excellent inhibitory effects on mycelial growth and spore germination of M. oryzae at 400 mg L . Liquid chromatography-tandem mass spectrometry was employed to analyze the components of the extracts, and heatmap clustering analysis with Mass Profiler Professional software revealed that lycorine and narciclasine may be the main active components. Lycorine and narciclasine, together with three other amaryllidaceous alkaloids (AAs), were then isolated from bulbs of Lycoris spp. Antifungal assays showed that lycorine and narciclasine had good inhibitory activities against M. oryzae in vitro, but the other three AAs showed no antifungal activities under test concentrations. In addition, lycorine and the ethyl acetate part of L. radiata showed good antifungal effects against M. oryzae in vivo, but narciclasine showed phototoxicity on rice when used alone.
Extracts of test Lycoris spp. and the main active component lycorine have excellent antifungal activities against M. oryzae, and are good candidates for developing control agents against M. oryzae. © 2023 Society of Chemical Industry.
稻瘟病由稻瘟病菌引起，是水稻最具破坏性的病害之一，由于常用控制剂产生抗性的问题，需要新型杀菌剂来控制稻瘟病。我们之前发现石蒜甲醇提取物对稻瘟病菌的菌丝生长具有极好的抑制作用，表明其具有开发稻瘟病菌控制剂的潜力。在本研究中，我们旨在研究不同石蒜属植物对稻瘟病菌的抑菌作用，并阐明主要的活性成分。
七种石蒜属植物鳞茎提取物在 400mg/L 时对稻瘟病菌的菌丝生长和孢子萌发具有极好的抑制作用。采用液相色谱-串联质谱法分析提取物的成分，用 Mass Profiler Professional 软件进行热图聚类分析显示，石蒜碱和去甲石蒜碱可能是主要的活性成分。然后从石蒜属植物鳞茎中分离出石蒜碱和去甲石蒜碱以及另外三种石蒜科生物碱（AAs）。抑菌试验表明，石蒜碱和去甲石蒜碱对稻瘟病菌具有良好的体外抑制活性，但在测试浓度下，另外三种 AAs 无抑菌活性。此外，石蒜碱和石蒜的乙酸乙酯部分对稻瘟病菌具有良好的体内抑菌作用，但去甲石蒜碱单独使用时对水稻具有光毒性。
测试的石蒜属植物提取物和主要活性成分石蒜碱对稻瘟病菌具有极好的抑菌活性，是开发稻瘟病菌控制剂的良好候选物。 © 2023 化学工业协会。
