Ordovas J M, Peterson J P, Santaniello P, Cohn J S, Wilson P W, Schaefer E J
USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA.
J Lipid Res. 1987 Oct;28(10):1216-24.
A noncompetitive enzyme-linked immunosorbent assay (ELISA) has been developed for measuring total plasma apolipoprotein (apo) B using affinity purified polyclonal and monoclonal antibodies. Microtiter plates from different manufacturers were tested with regard to their IgG binding characteristics; only one plate yielded consistent coefficients of variation of less than 5%. The optimal plasma dilution in this assay was 1:3000. IgG anti-apoB antisera conjugated to alkaline phosphatase was used as a second antibody. p-Nitrophenyl phosphate was utilized as substrate for color development, and the absorbance (410 nm) was read utilizing an ELISA reader interfaced with a microcomputer for data processing. Plasma apoB levels in plasma have been determined in 1115 male and female participants in the Framingham Offspring Study. Mean (+/- SD) plasma concentrations were 89 +/- 28 mg/dl. Significant age and sex related differences in apoB levels were noted.
已开发出一种非竞争性酶联免疫吸附测定法（ELISA），用于使用亲和纯化的多克隆和单克隆抗体测量血浆总载脂蛋白（apo）B。对不同制造商的微量滴定板进行了IgG结合特性测试；只有一种板的变异系数始终小于5%。该测定法的最佳血浆稀释度为1:3000。与碱性磷酸酶偶联的IgG抗apoB抗血清用作第二抗体。对硝基苯磷酸用作显色底物，并使用与微型计算机连接的ELISA读数器读取吸光度（410nm）以进行数据处理。在弗雷明汉后代研究的1115名男性和女性参与者中测定了血浆中的apoB水平。平均（±标准差）血浆浓度为89±28mg/dl。注意到apoB水平存在显著的年龄和性别相关差异。
