Department of Chemistry, College of Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
Department of Chemistry, College of Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
Talanta. 2023 May 15;257:124379. doi: 10.1016/j.talanta.2023.124379. Epub 2023 Feb 16.
A novel portable and disposable bipolar electrode (BPE)-electrochemiluminescence (ECL) device was fabricated for fumonisin B (FB) detection. BPE was fabricated by using MWCNTs and polydimethylsiloxane (PDMS) due to their excellent electrical conductivity and good mechanical stiffness. After the deposition of Au NPs on the cathode of BPE, the ECL signal could be improved 89-fold. Then a specific aptamer-based sensing strategy was constructed by grafting capture DNA on Au surface, followed by hybridizing with aptamer. Meanwhile, an excellent catalyst, Ag NPs was labeled on aptamer to activate oxygen reduction reaction, leading to a 13.8-fold enhancement in ECL signal at the anode of BPE. Under the optimal conditions, the biosensor exhibited a wide linear range of 0.10 pg/mL to 10 ng/mL for FB detection. Meanwhile, it demonstrated satisfactory recoveries for real sample detection with good selectivity, making it to be a convenient and sensitive device for mycotoxin assay.
一种新型的便携式一次性双极电极（BPE）-电化学发光（ECL）装置被用于黄曲霉毒素 B（FB）检测。BPE 是通过使用多壁碳纳米管（MWCNTs）和聚二甲基硅氧烷（PDMS）来制造的，因为它们具有优异的导电性和良好的机械刚度。在 BPE 的阴极上沉积 Au NPs 后，ECL 信号可以提高 89 倍。然后，通过在 Au 表面接枝捕获 DNA 构建了一种基于特定适体的传感策略，然后与适体杂交。同时，将一种优秀的催化剂 Ag NPs 标记在适体上，以激活氧还原反应，从而使 BPE 阳极的 ECL 信号增强 13.8 倍。在最佳条件下，该生物传感器对 FB 的检测表现出 0.10 pg/mL 至 10 ng/mL 的宽线性范围。同时，它对实际样品检测具有良好的选择性和令人满意的回收率，使其成为一种用于真菌毒素检测的方便和灵敏的装置。
