Mahmood Athraa A, Abbas Raghad Fadhil
Department of Oral Surgery and Periodontology, College of Dentistry, Mustansiriyah University, Baghdad, Iraq.
Department of Periodontology, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Eur J Dent. 2023 Oct;17(4):1338-1348. doi: 10.1055/s-0043-1761185. Epub 2023 Feb 22.
The nod-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome regulates the maturation and release of the cytokines as well as the activation of caspase in response to danger signals derived from pathogenic infection, tissue damage, andmetabolic changes that have a role in the pathogenesis of different diseases as periodontitis. Yet, the susceptibility to this illness could be determined by population-based genetic differences. The aim of this study was to determine whether periodontitis in Arab populations from Iraq is correlated with NLRP3 gene polymorphisms and measure clinical periodontal parameters and investigate their association with genetic polymorphisms of the NLRP3.
The study sample consisted of 94 participants ranging from 30 to 55 years old, both males and females who fulfilled the study's criteria. The selected participants were divided into two groups: the periodontitis group (62 subjects) and the healthy control group (32 subjects). The examination of clinical periodontal parameters of all participants was carried out, followed by a collection of venous blood for NLRP3 genetic analysis using the polymerase chain reaction-sequencing technique.
The genetic analysis of NLRP3 genotypes at four single nucleotide polymorphisms (SNPs) (rs10925024, rs4612666, rs34777555, and rs10754557), by Hardy-Weinberg equilibrium, identified nonsignificant differences in studied groups. The C-T genotype among periodontitis was significantly different from controls, while the C-C genotype among control was significantly different from periodontitis at NLRP3 rs10925024. Overall, there were 35 SNPs in the periodontitis group and 10 SNPs in the control group for rs10925024 with significant differences versus nonsignificant differences of the other SNPs between the studied groups. Clinical attachment loss and NLRP3 rs10925024 additionally demonstrated a significant positive correlation in the periodontitis subjects.
The findings suggested that polymorphisms of the gene may have a role and increasing the genetic susceptibility to periodontal disease in Arabs Iraqi patients.
含吡啉结构域的NOD样受体蛋白3（NLRP3）炎性小体可调节细胞因子的成熟与释放以及半胱天冬酶的激活，以应对源自病原体感染、组织损伤和代谢变化的危险信号，这些信号在不同疾病如牙周炎的发病机制中起作用。然而，这种疾病的易感性可能由基于人群的遗传差异决定。本研究的目的是确定伊拉克阿拉伯人群中的牙周炎是否与NLRP3基因多态性相关，测量临床牙周参数，并研究它们与NLRP3基因多态性的关联。
研究样本包括94名年龄在30至55岁之间的参与者，包括符合研究标准的男性和女性。选定的参与者分为两组：牙周炎组（62名受试者）和健康对照组（32名受试者）。对所有参与者进行临床牙周参数检查，随后采集静脉血，使用聚合酶链反应测序技术进行NLRP3基因分析。
通过哈迪-温伯格平衡对NLRP3基因在四个单核苷酸多态性（SNP）（rs10925024、rs4612666、rs34777555和rs10754557）处的基因型进行遗传分析，发现研究组之间无显著差异。在NLRP3 rs10925024处，牙周炎组中的C-T基因型与对照组有显著差异，而对照组中的C-C基因型与牙周炎组有显著差异。总体而言，对于rs10925024，牙周炎组有35个SNP，对照组有10个SNP，研究组之间该SNP的差异显著，而其他SNP的差异不显著。临床附着丧失与NLRP3 rs10925024在牙周炎受试者中还表现出显著的正相关。
研究结果表明，该基因的多态性可能起作用，并增加了伊拉克阿拉伯患者患牙周病的遗传易感性。
